Results 81 to 90 of about 2,528,058 (300)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Stability and economy analysis based on computational fluid dynamics and field testing of hybrid-driven underwater glider with the water quality sensor in Danjiangkou Reservoir

open access: yesAdvances in Mechanical Engineering, 2015
Hybrid-driven underwater glider is a new kind of unmanned platform for water quality monitoring. It has advantages such as high controllability and maneuverability, low cost, easy operation, and ability to carry multiple sensors.
Chao Li, Yanhui Wang, Zhiliang Wu
doaj   +1 more source

The role of lipid metabolism in neuronal senescence

open access: yesFEBS Open Bio, EarlyView.
Disrupted lipid metabolism, through alterations in lipid species or lipid droplet accumulation, can drive neuronal senescence. However, lipid dyshomeostasis can also occur alongside neuronal senescence, further amplifying tissue damage. Delineating how lipid‐induced senescence emerges in neurons and glial cells, and how it contributes to ageing and ...
Dikaia Tsagkari   +2 more
wiley   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

open access: yesFEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada   +5 more
wiley   +1 more source

Monitor and control equipment for the MV-3 mobile very long baseline interferometry station [PDF]

open access: yes
The system, designed around a commercially available process controller, is described. The hardware design, selection of the process control equipment, the design of the interface to other systems, and the analog monitor and process control assembly used
Sniffin, R. W.
core   +1 more source

The Brazilian experience in design for health: Interdisciplinary and Bioethics [PDF]

open access: yes, 2009
Health design in Brazil has been characterized historically by replacing imported products with others that are locally manufactured on a small scale. The formation of interdisciplinary groups has never been submitted to specific norms, particularly at ...
Bonatti, Fernanda Alves da Silva   +2 more
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

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