Results 31 to 40 of about 24,227 (257)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

Disinfection and reuse of N95 respirators during the COVID-19 pandemic

open access: yesRevista Cubana de Estomatología, 2022
Introduction: We are living in a context of COVID-19 pandemic, where the shortage of personal protective equipment in health is evident and it is expected that it will continue in the short and medium term.
Victor Marcel Aguilar Salas   +1 more
doaj  

Water reuse and recycling in Canada — history, current situation and future perspectives

open access: yesWater Cycle, 2020
Water reuse and recycling in Canada has been alive and viable where there is a need for water conservation or to reduce nutrients going to rivers and lakes.
Tony Van Rossum
doaj   +1 more source

Evaluating the effects of China's pollution controls on inter-annual trends and uncertainties of atmospheric mercury emissions [PDF]

open access: yesAtmospheric Chemistry and Physics, 2015
China's anthropogenic emissions of atmospheric mercury (Hg) are effectively constrained by national air pollution control and energy efficiency policies.
Y. Zhao   +3 more
doaj   +1 more source

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

open access: yesFEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks   +4 more
wiley   +1 more source

Reprocessing of single-use dental medical devices:analysis of chemical and physical impairment

open access: yesOnline Brazilian Journal of Nursing, 2009
<p class="MsoNormal" style="margin: 0in 0in 0pt; text-indent: 35.4pt; line-height: 200%; text-align: justify;"><span style="font-size: 10pt; line-height: 200%; font-family: ">The reprocessing of single-use dental-medical device </span> ...
Marina Celly Martins Ribeiro de Souza   +2 more
doaj  

The dangers of reused personal protective equipment: healthcare workers and workstation contamination

open access: yesJournal of Hospital Infection, 2022
Personal protective equipment (PPE) is essential to protect healthcare workers (HCWs). The practice of reusing PPE poses high levels of risk for accidental contamination by HCWs. Scarce medical literature compares practical means or methods for safe reuse of PPE while actively caring for patients.In this study, observations were made of 28 experienced ...
Doos, D.   +8 more
openaire   +4 more sources

Subclinical Optic Nerve Involvement in Radiologically Isolated Syndrome: Multimodal Detection and Diagnostic Impact

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objectives We aimed to determine the frequency of subclinical optic nerve (ON) lesions using MRI, optical coherence tomography (OCT), and visual evoked potentials (VEP) in radiologically isolated syndrome (RIS), and to assess their diagnostic and prognostic significance.
Christine Lebrun‐Frenay   +13 more
wiley   +1 more source

NFDI MatWerk Ontology (MWO): A BFO‐Compliant Ontology for Research Data Management in Materials Science and Engineering

open access: yesAdvanced Engineering Materials, EarlyView.
This article presents the NFDI‐MatWerk Ontology (MWO), a Basic Formal Ontology‐based framework for interoperable research data management in materials science and engineering (MSE). Covering consortium structures, research data management resources, services, and instruments, MWO enables semantic integration, Findable, Accessible, Interoperable, and ...
Hossein Beygi Nasrabadi   +4 more
wiley   +1 more source

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