Results 171 to 180 of about 1,492 (187)

Temperature-dependent responses of rats to ergovaline derived from endophyte-infected tall fescue

Journal of Animal Science, 1995
Ergovaline, found in Acremonium coenophialum-infected tall fescue, is considered to be responsible for many symptoms associated with fescue toxicosis. Rats were tested to determine time-related thermoregulatory responses to acute treatment with ergovaline during specific thermal challenges. Isolated ergovaline was administered to rats (15 micrograms/kg
D E, Spiers, Q, Zhang, P A, Eichen, G E, Rottinghaus, G B, Garner, M R, Ellersieck   +5 more
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Improved Extraction and HPLC Methods for Ergovaline from Plant Material and Rumen Fluid

Journal of Veterinary Diagnostic Investigation, 1994
Ergovaline, the main ergopeptine alkaloid produced in tall fescue infected with Acremonium coenophialum, is known to cause tall fescue toxicosis. Current methods in use for quantifying ergovaline in plant material have several disadvantages, including large solvent volumes and long analysis time.
A M, Cràig, D, Bilich, J T, Hovermale, R E, Welty   +3 more
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DETERMINATION OF ERGOVALINE IN ENDOPHYTED SEEDS BY HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY (HPTLC)

Journal of Liquid Chromatography & Related Technologies, 2000
High performance thin layer chromatography (HPTLC) is used to rapidly determine the presence and amount of ergovalin in seeds of Graminaceae infested with Neotyphodium Coenophialum. Quinine is used as an internal standard. The mycotoxin is solubilized under acid conditions and extracted with chloroform under alkaline conditions.
Videmann, B., Bony, Sylvie, Berny, Philippe   +2 more
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Ergovaline-induced vasoconstriction in an isolated bovine lateral saphenous vein bioassay.

Journal of animal science, 2007
Ergovaline has been proposed as a toxic component of endophyte-infected tall fescue. As many of the symptoms of fescue toxicosis are a result of compromised circulation, the objective of this study was to examine the vasoconstrictive potentials of ergovaline and a more documented ergopeptine, ergotamine, using a bovine, lateral (cranial branch ...
J L, Klotz, L P, Bush, D L, Smith, W D, Shafer, L L, Smith, B C, Arrington, J R, Strickland   +6 more
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Bioaccumulation of ergovaline in bovine lateral saphenous veins in vitro.

Journal of animal science, 2009
Ergot alkaloids have been associated with vasoconstriction in grazing livestock affected by the fescue toxicosis syndrome. Previous in vitro investigations studying how ergot alkaloids caused vasoconstriction have shown that ergovaline has a distinct receptor affinity and sustained contractile response. A similar contractile response has not been noted
J L, Klotz, B H, Kirch, G E, Aiken, L P, Bush, J R, Strickland   +4 more
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Serotonin can stimulate vasorelaxation in ovine lateral saphenous veins precontracted with ergovaline

Journal of Animal Science
Abstract The ergot alkaloid ergovaline is a potent vasoconstrictor. Previous research has shown that ergovaline can bind serotonin (5-HT) receptors and elicit vasoconstrictive activity. Three experiments were conducted to evaluate 5-HT as a vasorelaxant in the ovine saphenous artery and vein. Blood vessels were collected from mixed-breed
James L Klotz, Celina M Checura, Maslyn A Greene, Huihua Ji, Susan K Duckett   +4 more
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Ergovaline does not alter the severity of ryegrass staggers induced by lolitrem B

New Zealand Veterinary Journal, 2018
To investigate a possible interaction between lolitrem B and ergovaline by comparing the incidence and severity of ryegrass staggers in sheep grazing ryegrass (Lolium perenne) containing lolitrem B or ryegrass containing both lolitrem B and ergovaline.Ninety lambs, aged approximately 6 months, were grazed on plots of perennial ryegrass infected with ...
SC Finch, JB Vlaming, BL Sutherland, C van Koten, WJ Mace, LR Fletcher   +5 more
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Analysis of ergovaline in milk using high-performance liquid chromatography with fluorimetric detection

Journal of Chromatography B: Biomedical Sciences and Applications, 1999
A high-performance liquid chromatographic method for the determination of the mycotoxin ergovaline in goat's milk is described here. Ergotamine was used as an internal standard. For a sample size of 5.0 ml, the cleanup method included precipitation of milk protein with acetone.
Durix, A., Jaussaud, P., Garcia, P., Bonnaire, Y., Bony, Sylvie   +4 more
openaire   +3 more sources

Thermoregulatory Effects of Ergovaline Isolated from Endophyte-Infected Tall Fescue Seed on Rats

Journal of Agricultural and Food Chemistry, 1994
Ergovaline (EV) was isolated from Acremonium coenophialum-infected tall fescue seed. Seed (2 kg) was extracted with 20 L of 5% lactic acid in water/methanol (4:1), filtered, and passed over an Amberlite XAD-2 column. Ergovaline was eluted with MeOH, concentrated, and chromatographed on two reversed-phase C 18 columns (purity >95%, 40% overall recovery)
Qinggen Zhang, Donald E. Spiers, George E. Rottinghaus, George B. Garner   +3 more
openaire   +1 more source

ERGOVALINE TOXICITY ON CACO-2 CELLS AS ASSESSED BY MTT, ALAMARBLUE, AND DNA ASSAY

In Vitro Cellular and Developmental Biology-Animal, 2003
The exact mechanisms of fescue toxicity in animals have yet to be established, but it has been associated with an inability to thrive. Ergovaline is the major ergopeptine alkaloid associated with fungal infections of tall fescue. Gastrointestinal (GI) toxicity of ergovaline (10(-11) to 10(-4) M) was evaluated in Caco-2 cells (mimicking the GI ...
openaire   +2 more sources