Results 61 to 70 of about 551,016 (266)

Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition

FEBS Open Bio, EarlyView.
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima, Shogo Okazaki, Muneaki Tamura, Shuichi Sato, Kenichi Imai   +4 more
wiley   +1 more source

Ethanol production from carrot by-products

Revista de Ciências Agrárias, 2019
A cenoura (Daucus carota L.), planta da família das umbelíferas, caracteriza-se como uma das mais importantes olericulturas. Os subprodutos dos lavadores e das indústrias processadoras de cenoura podem ser valorizados com fins alimentares ou para obtenção de extratos para posterior utilização.
Passos, Flávia Regina, Alves, Lucélia Cristina, Mendes, Fabrícia Queiroz, Rocha, Kátia Rodrigues de Oliveira, Ribeiro, Lorena, Pereira, Samarina Gabrielle de Fátima, Silva, Thaís Oliveira da, Alcântara, Válter de Castro   +7 more
openaire   +1 more source

Metformin promotes mitochondrial integrity through AMPK‐signaling in Leber's hereditary optic neuropathy

FEBS Open Bio, EarlyView.
Metformin mediates mitochondrial quality control in Leber's hereditary optic neuropathy (LHON) fibroblasts carrying mtDNA mutations. At therapeutic levels, metformin activates AMPK signaling to restore mitochondrial dynamics by promoting fusion and restraining fission, while preserving mitochondrial mass, enhancing autophagy/mitophagy and biogenesis ...
Chatnapa Panusatid, Rapasviranda Soiyangsuk, Maneeluck Tanadjindarat, Chayanon Peerapittayamongkol   +3 more
wiley   +1 more source

Optimizing ethanol production selectivity

Mathematical and Computer Modelling, 2011
Lactococcus lactis metabolizes glucose homofermentatively to lactate. However, after disruption of the gene coding for lactate dehydrogenase, LDH, a key enzyme in NAD^+ regeneration, the glycolytic flux shifts from homolactic to mixed-acid fermentation with the redirection of pyruvate towards production of formate, acetate, ethanol and CO"2.
Lall, Raman, Donohue, Timothy J., Marino, Simeone, Mitchell, Julie C.   +3 more
openaire   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Selective Electroreduction of CO2 to Ethanol: Mechanistic Understanding and Catalyst Development

MetalMat
The electrochemical reduction of carbon dioxide (CO2RR) has garnered significant attention as a promising strategy for producing carbon‐neutral fuels and chemicals using electricity.
Suwon Rhi, Sungwook Park, Do Yeon Heo, Soshi Iimura, Soo Young Kim   +4 more
doaj   +1 more source

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

FEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold, Janik Riese, Lancelot Seillier, Mark Kühnel, Julia Pannhausen, Charlotte O. J. Fröhlich, Christian Martin, Peter Boor, Matthias Saar, Danny D. Jonigk, Nadine T. Gaisa, Michael Rose   +11 more
wiley   +1 more source

Transcriptional Regulation Mechanisms in Adaptively Evolved Pichia kudriavzevii Under Acetic Acid Stress

Journal of Fungi
Acetic acid, a common weak acid in industrial fermentation processes, occurs naturally in lignocellulosic hydrolysates and is a byproduct of microbial metabolism.
Sureeporn Dolpatcha, Huynh Xuan Phong, Sudarat Thanonkeo, Preekamol Klanrit, Nongluck Boonchot, Mamoru Yamada, Pornthap Thanonkeo   +6 more
doaj   +1 more source

Improvement of ethanol production in Saccharomyces cerevisiae by high-efficient disruption of the ADH2 gene using a novel recombinant TALEN vector

Frontiers in Microbiology, 2016
Bioethanol is becoming increasingly important in energy supply and economic development. However, the low yield of bioethanol and the insufficiency of high-efficient genetic manipulation approaches limit its application.
Wei Ye, Weimin Zhang, Taomei Liu, Guohui Tan, Haohua Li, Zilei Huang   +5 more
doaj   +1 more source