Results 21 to 30 of about 159,255 (285)
Journal of Medical Microbiology and Infectious Diseases, 2022 Introduction: In the past decade, multidrug-resistant Acinetobacter baumannii has become one of the most critical challenges in treating infected patients. The AdeABC efflux pump is the most important among the various resistance mechanisms. This pump
Amin Sepehr1 +2 more
doaj +1 more source
Methods and Protocols, 2023 We describe a workflow for efficient, environmentally attentive, and sustainable practices related to routine agarose gel electrophoresis. The methods reduce plastic waste and improve efficiency, especially for the exhaustive screening of difficult-to ...
Nadeem Asad +4 more
doaj +1 more source
Flow Cytometric Analysis of Efflux by Dye Accumulation
Frontiers in Microbiology, 2019 Gram-negative infections are increasingly difficult to treat because of their impermeable outer membranes (OM) and efflux pumps which maintain a low intracellular accumulation of antibiotics within cells.
Emily E. Whittle +5 more
doaj +1 more source
Probing the Structure of DNA Aptamers with a Classic Heterocycle.
Molecules, 2004 DNA aptamers are synthetic, single-stranded DNA oligonucleotides selectedby SELEX methods for their binding with specific ligands. Here we present ethidiumbinding results for three related DNA aptamers (PDB code: 1OLD, 1DB6, and 2ARG)that bind L ...
G. Reid Bishop, Arthur E. Wood IV
doaj +1 more source
Curcumin decreases astrocytic reaction after gliotoxic injury in the rat brainstem
Arquivos de Neuro-PsiquiatriaRecent studies have demonstrated that curcumin (Cur) has antioxidant, anti-inflammatory and anti-fibrotic effects. Ethidium bromide (EB) injections into the central nervous system (CNS) are known to induce local oligodendroglial and astrocytic loss ...
Eduardo Bondan +2 more
doaj +1 more source
Propentofylline reduces glial scar development following gliotoxic damage in the rat brainstem
Arquivos de Neuro-PsiquiatriaPropentofylline is a xanthine derivative that depresses activation of glial cells, whose responses contribute to neural tissue damage during inflammation.
Eduardo Fernandes Bondan +6 more
doaj +1 more source
Fluorescence microscopy methods for the determination of somatic cell count in raw cow's milk
Veterinární Medicína, 2016 The direct fluorescence microscopy method with ethidium bromide staining can be used for somatic cell counting in raw cow's milk. However, this method has some limitations that may influence the results of the analysis.
P. Zajac +3 more
doaj +1 more source
Caffeine-induced release of small molecules from DNA nanostructures
iScience, 2023 Summary: Several planar aromatic molecules are known to intercalate between base pairs of double-stranded DNA. This mode of interaction has been used to stain DNA as well as to load drug molecules onto DNA-based nanostructures.
Bharath Raj Madhanagopal +3 more
doaj +1 more source
SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Synthesis and Neuropharmacological Evaluation of Some Novel Quinoxaline 2, 3-Dione Derivatives
The Scientific World Journal, 2012 Quinoxaline-2, 3-dione obtained from cyclocondensation reaction of o-phenylene diamine with oxalic acid was reacted with three different ketones and formaldehyde to give the corresponding Mannich bases in satisfactory yield.
Selvaraj Jubie +2 more
doaj +1 more source