Results 71 to 80 of about 147,555 (214)

Protocol for high-sensitivity/long linear-range spectrofluorimetric DNA quantification using ethidium bromide

open access: yesBioTechniques, 2007
Ethidium bromide (EtBr) is the most widely used fluorescent dye in nucleic acid gel electrophoresis since decades. However, it has been essentially forgotten in DNA quantification by spectrofluorimetry.
Veronica Bonasera   +2 more
doaj   +1 more source

Vat Polymerization 3D Printing of Hydrogels With Tunable Porosity

open access: yesSmall Methods, EarlyView.
Nanodroplet emulsion templating enables DLP printing of porous hydrogels with pore sizes tunable across two orders of magnitude, from 46.15 to 0.66 µm. The printed interconnected networks retain high fidelity while improving mechanical robustness and cellular responses, providing a versatile platform for porous hydrogel biofabrication.
Liwei Liu   +13 more
wiley   +1 more source

Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison

open access: yesGenetics and Molecular Biology, 2009
The present study applied the comet assay to erythrocytes of Oreochromis niloticus with the aim of improving protocols to detect DNA damage in these cells, by using two distinct pHs (pH = 12.1 and pH > 13) and evaluating whether there is a correspondence
Cintya A. Christofoletti   +2 more
doaj  

Ameliorating the Effects of Trypanosoma Evansi Infection in Wistar Rats Using Selected Nutraceuticals, Ethidium Bromide and Ethidium Chloride

open access: yesUMYU Scientifica Journal
African Trypanosomiasis is a disease that affects humans and animals, caused by trypanosomes in sub-Saharan Africa, despite decades of vector control and treatments. The disease remains endemic and an impediment to socio-economic development.
Yahaya Shuaibu   +2 more
doaj   +1 more source

MSDS of Ethidium Bromide

open access: yes, 2016
The Materials Safety Data Sheet (MSDS) for Ethidium Bromide, a substance commonly used for DNA intercalation fluorescence marking.
openaire   +1 more source

Cytogenotoxic and Molecular Assessment of Pyrethrins on Non‐Target Allium cepa Roots

open access: yesEnvironmental Toxicology, EarlyView.
ABSTRACT The pyrethrins, which form a part of naturally occurring insecticidal chemicals derived from chrysanthemum flowers, not only provide rapid knockdown of a broad spectrum of insect pests but are also widely used in integrated pest management due to a generally favorable safety profile, largely attributed to low mammalian toxicity.
Yudum Yeltekin Uğur   +2 more
wiley   +1 more source

Effects of Roscovitine and Serum Starvation on the Viability and G0/G1 Synchronization in Somatic Cells from the Antillean Manatee (Trichechus manatus manatus, Linnaeus 1758)

open access: yesZoo Biology, EarlyView.
ABSTRACT The success of nuclear reprogramming techniques, including the G0/G1 synchronization of somatic cells, depends on species‐specific optimization. While roscovitine and serum starvation have been successfully applied in domestic and wild terrestrial mammals, their use in aquatic mammals remains poorly investigated.
Luanna Lorenna Vieira Rodrigues   +10 more
wiley   +1 more source

Applying molecular dynamics simulations to validate OCT1 substrates uncovers vitamin K1 as a high-affinity ligand

open access: yesFrontiers in Pharmacology
OCT1 is a hepatic membrane uptake transporter of clinical importance in drug metabolism and pharmacokinetics. OCT1 exhibits broad substrate specificity, mediating uptake of diverse compounds, including the neurotransmitter serotonin and the antidiabetic ...
Elham Omer Mahgoub   +3 more
doaj   +1 more source

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