Results 101 to 110 of about 241,573 (255)

A continuous kinetic assay for RNA-cleaving deoxyribozymes, exploiting ethidium bromide as an extrinsic fluorescent probe [PDF]

open access: yes, 2002
We describe a rapid and inexpensive method to monitor the kinetics of small RNA-cleaving deoxyribozymes, based on the exogenous fluorophore ethidium bromide.
FERRARI, Davide, PERACCHI, Alessio
core   +1 more source

Cytogenotoxic and Molecular Assessment of Pyrethrins on Non‐Target Allium cepa Roots

open access: yesEnvironmental Toxicology, EarlyView.
ABSTRACT The pyrethrins, which form a part of naturally occurring insecticidal chemicals derived from chrysanthemum flowers, not only provide rapid knockdown of a broad spectrum of insect pests but are also widely used in integrated pest management due to a generally favorable safety profile, largely attributed to low mammalian toxicity.
Yudum Yeltekin Uğur   +2 more
wiley   +1 more source

Fluorometric determination of ethidium bromide efflux kinetics in Escherichia coli

open access: yesJournal of Biological Engineering, 2009
Background Efflux pump activity has been associated with multidrug resistance phenotypes in bacteria, compromising the effectiveness of antimicrobial therapy.
Monteiro Gabriel A   +9 more
doaj   +1 more source

Intercalation of ethidium bromide into a triple-stranded oligonucleotide

open access: yesNucleic Acids Research, 1991
We have examined the ability of a cationic planar chromophore, ethidium bromide, to intercalate into a short, defined triple helix. Using UV absorption, fluorescence spectroscopy and a gel retardation assay we demonstrate that ethidium bromide is able to bind to a triple helix with a lower affinity than to the corresponding duplex. Energy transfer from
J L, Mergny   +4 more
openaire   +3 more sources

Effects of Roscovitine and Serum Starvation on the Viability and G0/G1 Synchronization in Somatic Cells from the Antillean Manatee (Trichechus manatus manatus, Linnaeus 1758)

open access: yesZoo Biology, EarlyView.
ABSTRACT The success of nuclear reprogramming techniques, including the G0/G1 synchronization of somatic cells, depends on species‐specific optimization. While roscovitine and serum starvation have been successfully applied in domestic and wild terrestrial mammals, their use in aquatic mammals remains poorly investigated.
Luanna Lorenna Vieira Rodrigues   +10 more
wiley   +1 more source

Reversible and irreversible pollutant-induced bacterial cellular stress effects measured by ethidium bromide uptake and efflux.

open access: yes, 2012
Chemical pollution is known to affect microbial community composition but it is poorly understood how toxic compounds influence physiology of single cells that may lay at the basis of loss of reproductive fitness.
Czechowska, K.   +3 more
core   +1 more source

A cryptic targeting signal creates a mitochondrial FEN1 isoform with tailed R-Loop binding properties.

open access: yesPLoS ONE, 2013
A growing number of DNA transacting proteins is found in the nucleus and in mitochondria, including the DNA repair and replication protein Flap endonuclease 1, FEN1. Here we show a truncated FEN1 isoform is generated by alternative translation initiation,
Lawrence Kazak   +6 more
doaj   +1 more source

A Novel Antioxidant Peptide Cath‐Np Derived from Nanorana parkeri Attenuates UVB‐Induced Skin Photodamage in Mice

open access: yesIntegrative Zoology, EarlyView.
Schematic representation of Cath‐Np as an antioxidant suppressing acute skin damage caused by ultraviolet B (UVB) radiation in mice. Cath‐Np inhibits the activation of p53 and mitogen‐activated protein kinase pathways while it activates the Nrf2 pathway by its strong antioxidant effects, and accordingly suppresses the expression of inflammatory factors
Hang Liao   +8 more
wiley   +1 more source

Reversible and Irreversible Pollutant-Induced Bacterial Cellular Stress Effects Measured by Ethidium Bromide Uptake and Efflux

open access: yes, 2016
Chemical pollution is known to affect microbial community composition but it is poorly understood how toxic compounds influence physiology of single cells that may lay at the basis of loss of reproductive fitness.
Jan Roelof van der Meer (152275)   +1 more
core   +1 more source

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