Results 51 to 60 of about 147,555 (214)

Assessment of In Vitro Nano‐Cytotoxicity Through Fluorescence Microscopy via Neural Network‐Based Live/Dead Cell Classification

Advanced Intelligent Systems, EarlyView.
FluoAI is a two‐stage, label‐free method for quantifying nanomaterial‐induced cytotoxicity from conventional fluorescence microscopy images. Mask R‐CNN segmentation and DenseNet‐121 classification provide rapid live/dead classification matching human accuracy.
Ugur C. Topkiran, Ali Gasimli, Lal Durmaz, Diya Vashani, Himish Paul, Giridhar R. Akkaraju, Anton V. Naumov   +6 more
wiley   +1 more source

Investigating the Removal Ethidium Bromide from Aqueous Solutions using Activated Persulfate in Electrochemical Process

پژوهان, 2018
Background & Objectives: Ethidium bromide is known as a Mutagenic substance that due to some of its characteristics including accumulative property in organism bodies, can cause major environmental problems.
Mohammad Reza Samarghandi, Yousef Poureshgh, Mohammad Vanaei Tabar, Zahra Rahmani, Sattar Ahmadi   +4 more
doaj  

DNA extraction from formalin-fixed tissue: new light from the deep sea

Scientia Marina, 2010
DNA samples were extracted from ethanol and formalin-fixed decapod crustacean tissue using a new method based on Tetramethylsilane (TMS)-Chelex. It is shown that neither an indigestible matrix of cross-linked protein nor soluble PCR inhibitors impede PCR
Ferran Palero, Sally Hall, Paul F. Clark, David Johnston, Jackie Mackenzie-Dodds, Sven Thatje   +5 more
doaj   +1 more source

Scalable Separation and Purification of M13‐Bacteriophage‐Derived Particles Containing Artificial Single‐Stranded DNA Produced by Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Single‐stranded DNA (ssDNA) solutions are promising innovations shaping the future of genetic research and medical therapies. In principle, biotechnological mass production of ssDNA can be achieved using Escherichia coli in a high‐cell density fed‐batch process by secreting phagemid particles derived from the filamentous M13 bacteriophages ...
Nathalie Hafner, Wiebke Winkler, Maximilian N. Honemann, Hendrik Dietz, Dirk Weuster‐Botz   +4 more
wiley   +1 more source

Voltage‐programmed capillary gel electrophoresis enables rapid detection of bovine coronavirus DNA

Bulletin of the Korean Chemical Society, EarlyView.
A voltage‐programmed capillary gel electrophoresis method enables approximately 20‐fold faster analysis of bovine coronavirus compared with conventional slab gel electrophoresis. Abstract Bovine coronavirus (BCoV) is a major pathogen that causes enteric and respiratory disease in cattle and considerable economic losses in the livestock industry.
Hyunjin Jung, Jihyun Yun, Jinho Park, Seong Ho Kang   +3 more
wiley   +1 more source

Zaleya decandra exerts anti-cancer activity in HCT-116 cancer cells by impairing mitochondrial activity

Bangladesh Journal of Pharmacology, 2019
An attempt has been made to investigate the role of Zaleya decandra on the survival of HCT colon cancer cells in vitro. The cell viability and apoptosis were assessed using MTT assay and acridine orange/ethidium bromide staining, respectively ...
Malarvizhi Deivasigamani, Ajay Kasivishwanathan Chandrasekar, Sridhar Muthusami, Gopalakrishnan Velliyur Kanniappan, Hariprasath Lakshmanan   +4 more
doaj   +1 more source

Lean ZSF1 rats in basic research on heart failure with preserved ejection fraction

ESC Heart Failure, Volume 12, Issue 2, Page 1474-1478, April 2025.
Abstract Aims ZSF1 obese rats harbouring two mutant leptin receptor alleles (Leprcp and Leprfa) develop metabolic syndrome and heart failure with preserved ejection fraction (HFpEF), making them a widely used animal model in cardiometabolic research.
Petra Büttner, Antje Augstein, Mahmoud Abdellatif, André Lourenço, Adelino Leite‐Moreira, Inês Falcão‐Pires, Sarah Werner, Holger Thiele, Simon Sedej, Antje Schauer, Volker Adams   +10 more
wiley   +1 more source

GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps

Antibiotics, 2020
Considering the evidence that essential oils, as well as safrole, could modulate bacterial growth in different resistant strains, this study aims to characterize the phytochemical profile and evaluate the antibacterial and antibiotic-modulating ...
Ray S. Almeida, Priscilla R. Freitas, Ana Carolina J. Araújo, Irwin R. Alencar Menezes, Eduardo L. Santos, Saulo R. Tintino, Talysson F. Moura, Jaime R. Filho, Vitória A. Ferreira, Ana Cristina A. Silva, Luiz E. Silva, Wanderlei do Amaral, Cícero Deschamps, Marcello Iriti, Henrique D. Melo Coutinho   +14 more
doaj   +1 more source

Genetic variants of EcPV2 E6 and E7 in equine genital and oronasal squamous cell carcinoma

Equine Veterinary Journal, EarlyView.
Abstract Background Since its discovery in 2008, equine papillomavirus type 2 (EcPV2) has been detected in up to 100% of equine genital and up to 40% of equine oronasal squamous cell carcinomas (SCCs). Preliminary sequence data point to the existence of EcPV2 E6 and E7 oncogene variants compared to the British reference sequence from 2008.
Maya Jenner, Sabine Brandt
wiley   +1 more source

Antimicrobial Sensitivity Profile and Resistance Gene Research in Escherichia coli Isolated From Water and Fish From Lagoa da Conceição, Florianópolis, SC, Brazil

Food Safety and Health, EarlyView.
This paper highlights a concerning scenario of environmental contamination by multidrug‐resistant bacteria, underscoring the need for integrated surveillance and control strategies to address antimicrobial resistance in aquatic environments within the context of One Health.
Francisco Lucas de Amorim Nascimento, Luan Amaral Alexandre, Cynthia Farias Manassi, Alice Cristina da Silva, Luísa Aimée Schmitt Lobe, Gustavo Rocha, Mateus Rocha Ribas, Victor Wolleck, Gabriele Caroline Gottschalk de Abreu, Alessandra Valéria Vortmann, Sheila Mello da Silveira, Rodrigo Barcellos Hoff, Jussara Kasuko Palmeiro, Silvani Verruck   +13 more
wiley   +1 more source