Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Mental health in the Sámi population and the importance of cultural affiliation: cross-sectional analyses at two time points in mid and Northern Norway. [PDF]
Helander L, Silviken A, Heir T.
europepmc +1 more source
Hydrostatic pressure activates HIF‐1α via β‐catenin to promote stemness in breast cancer cells
To mimic the elevated intestinal fluid pressure in breast cancers, we loaded human breast cancer cells (MCF‐7, MDA‐MB‐453, and BT‐474) to 50 mmHg hydrostatic pressure. Hydrostatic pressure exposure upregulated HIF‐1α and induced stemness in MCF‐7 and BT‐474 cells.
Da Zhai +8 more
wiley +1 more source
Mental health problems among ethnic minorities during public crises: a systematic review of coping strategies during the COVID-19 pandemic. [PDF]
Sharif N, Chen W, Niu M.
europepmc +1 more source
Digital Resources and Social Connectedness Among Ethnic Minority Older Adults: Systematic Review and Meta-Analysis. [PDF]
Amoah PA +4 more
europepmc +1 more source
Exploring spatial heterogeneity and influencing factors of cultural inheritance level in mountain traditional villages: a case of Leishan County. [PDF]
Wei H, Fan L, Wu C.
europepmc +1 more source
The Down Low Among Latino Men Who Have Sex With Men: A Review and Theoretical Analysis. [PDF]
Head KR.
europepmc +1 more source
Promoting a Sense of Belonging Among Care-Experienced Young People: Insights From a Scoping Review. [PDF]
Critchlow M, John M, Dunn A.
europepmc +1 more source
An exploration of the structure and understanding of <i>Xin</i> ("") in Chinese culture: the development of a theoretical model and questionnaire instruments. [PDF]
Lin L, Jing D, Sun C.
europepmc +1 more source

