Results 241 to 250 of about 986,878 (293)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

SIRT4 positively regulates autophagy via ULK1, but independently of HDAC6 and OPA1

FEBS Open Bio, EarlyView.
Cells expressing SIRT4 (H161Y), a catalytically inactive mutant of the sirtuin SIRT4, fail to upregulate LC3B‐II and exhibit a reduced autophagic flux under stress conditions. Interestingly, SIRT4(H161Y) promotes phosphorylation of ULK1 at S638 and S758 that are associated with inhibition of autophagy initiation.
Isabell Lehmkuhl, Khawar Amin, Lydia Gabriel, Nils Hampel, Afshin Iram, Julia Hesse, Constanze Wiek, Jasmin Thuy Vy Nguyen, Helmut Hanenberg, Jürgen Scheller, M. Reza Ahmadian, Björn Stork, Doreen M. Floss, Roland P. Piekorz   +13 more
wiley   +1 more source

Ethno racial issues in pharmaceutical education: a curricular analysis of brazilian federal higher education institutions. [PDF]

BMC Med Educ
Cavalcante NC, de Castro Araújo-Neto F, Dos Santos MR, Mesquita AR, Lyra DP.   +4 more
europepmc   +1 more source

Metformin promotes mitochondrial integrity through AMPK‐signaling in Leber's hereditary optic neuropathy

FEBS Open Bio, EarlyView.
Metformin mediates mitochondrial quality control in Leber's hereditary optic neuropathy (LHON) fibroblasts carrying mtDNA mutations. At therapeutic levels, metformin activates AMPK signaling to restore mitochondrial dynamics by promoting fusion and restraining fission, while preserving mitochondrial mass, enhancing autophagy/mitophagy and biogenesis ...
Chatnapa Panusatid, Rapasviranda Soiyangsuk, Maneeluck Tanadjindarat, Chayanon Peerapittayamongkol   +3 more
wiley   +1 more source

Accessing Care Across Cultures: Qualitative Insights Into the Reality of Informal Caregivers From Ethnically Minoritised Groups. [PDF]

Health Expect
Robinson-Barella A, Wayoe T, Deakin RL, Richardson CL.   +3 more
europepmc   +1 more source

Engineering tandem VHHs to target different epitopes to enhance antibody‐dependent cell‐mediated cytotoxicity

FEBS Open Bio, EarlyView.
Tandem VHH targeting distinct EGFR epitopes were engineered into a monovalent bispecific antibody (7D12‐EGA1‐Fc) with more potent ADCC without increasing affinity to EGFR. Structural modeling of 7D12‐EGA1‐Fc showed cross‐linking of separate EGFR domains to enhance CD16a engagement on NK cells.
Yuqiang Xu, Hao Jiang, Limin Chen, Fulai Zhou, Ying Jin, Mark L. Chiu   +5 more
wiley   +1 more source

Cultural competency wheel: advancing a model to characterize the domains and attributes of cultural competency from published nursing literature via integrative review. [PDF]

BMC Nurs
Skaria R, Rivero JA, Dino MJ.
europepmc   +1 more source

Improving treatment education in Black and South Asian women. [PDF]

Support Care Cancer
Patel A, Steventon L, McLean J, Nabhani-Gebara S, Kılıç A, Jani YH, Moon Z, Chambers P.   +7 more
europepmc   +1 more source

From appraisal to word-of-mouth: affective pathways through awe and restoration in high-altitude ecocultural tourism using a mixed-methods design. [PDF]

Front Psychol
Liao W, Khan MS, Liao G.
europepmc   +1 more source

Aging in Nationhood: Everyday Nationalism and Belonging Among Seniors in Old-Age Homes in Québec. [PDF]

Br J Sociol
Stallone J.
europepmc   +1 more source