Results 111 to 120 of about 632,939 (308)
Constituting European Citizenship
Democratic TheoryAbstract The constituted legal status of “Union citizenship” has added another democratic static to the European Union's institutional architecture but it is not yet a status of full political empowerment. What is missing is a citizen-centered opening-up of the (technocratically disguised) European level as a political arena.
openaire +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
A Treaty of the Constitution for Europe and the starting points for the "European criminal law"
Časopis pro Právní Vědu a Praxi, 2006 The following conveyance is based on an extensive worked out pilot study "A dimension of the questions of the criminal justice of the Treaty of the Constitution for Europe and its importance for European criminal law (Europeanization of the Czech penal ...
Vladimír Kratochvíl
doaj
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
Czech Journal of International Relations, 2005 This article focuses on the Presidency of the Council of Ministers of the European Union (EU). It aims to discern the different possibilities for fulfilling the Presidency's functions after the adoption of the so-called European constitution.
Markéta Pitrová, Petr Kaniok
doaj
Digital twins to accelerate target identification and drug development for immune‐mediated disorders
FEBS Open Bio, EarlyView.Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley +1 more source
German Law JournalThis Article examines the evolving concept of the material constitution and its application to the EU. The first part focuses on the theory of Italian jurist Costantino Mortati, exploring both its foundations and its contemporary relevance.
Leonardo Ravaioli
doaj +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source