Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Application of the funding GAP method in the process of financing regional development in Romania [PDF]
The present paper aims at analyzing the framework and the methodology for applying the funding gap method in order to determine the level of structural funds co-financing in the process of financing regional development in Romania, through the Regional ...
Oana GHERGHINESCU
core
FEBS Open Bio, EarlyView.Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
Innovation policy in the European Union: instruments and objectives [PDF]
We provide an overview of the specific innovation policies that are implemented at European level, highlighting, where possibile, the connections between these policies and the guidance documents issued by the Community’s institutions.
Rossi, Federica
core +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
European Money at Work: Contracting a European Identity? [PDF]
Most literature on the interaction between European institutions and NGOs focuses on interest representation and lobbying. This article tackles a neglected aspect of this interaction: the effects of European funding opportunities on NGO's organizational ...
Rosa Sanchez Salgado
core
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
European Structural and Investment Funds vs European Funds Allocated through the National Recovery and Resilience Program [PDF]
Finanţe: Provocările viitorului, 2022 Dumitru BELDIMAN +2 more
doaj
The performance evaluation of hedge funds: a comparison of different approaches using European data [PDF]
The standard approach to the evaluation of funds assumes a normal return distribution and uses the variance as a measure of the funds risk. A few characteristics of hedge funds, such as the remuneration mechanism of the portfolio manager, make this ...
Carretta, Alessandro +1 more
core +1 more source