Results 101 to 110 of about 15,758,896 (298)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Exploring misery discourses: problematized Roma in labour market projects

open access: yesEuropean Journal for Research on the Education and Learning of Adults, 2016
The aim of this article is to analyse learning practices in labour market projects cofinanced by the European Social Fund (ESF) targeting unemployed Roma in Sweden.
Viktor Vesterberg
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

The Role of European Employment Strategy in Knowledge Transfer Based on the Example of Borsod-Abaúj-Zemplén County

open access: yesTheory, Methodology, Practice, 2002
In the first part of the essay I am dealing with the achievements, concerning employment, of the European Union, in the second part I am trying to answer the question whether to what extent it is possible to utilise the knowledge and expertise ...
Gizella Sikora Tóthné
doaj  

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Returns on EU Funding for Human Capital Development in Rural Areas [PDF]

open access: yesRevista de Management Comparat International, 2015
The paper aims to explore at which extent the investments in human capital in rural areas could contribute to rural development and competitiveness. European Union is investing substantial resources for faster growth and competitiveness, addressing the ...
Laura Elena MARINAS
doaj  

Who makes European Cohesion Policy: a practitioners’ learning perspective

open access: yesRegional Studies, Regional Science, 2016
Through the European Social Fund (ESF), the European Union subsidizes social inclusion and occupational trainee projects. This policy instrument is formulated by European Union institutions and implemented by regions through a call for projects which ...
Fanny Sbaraglia
doaj   +1 more source

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

open access: yesFEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

open access: yesFEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Central and eastern european social model [PDF]

open access: yes
The 10 former communist countries from Central and Eastern Europe have inherited similar social protection systems, specific to an ethatist economy, and faced similar issues along the transition to market economy. Distinct in Europe through common traits,
Rotaru, Marius-Petre
core  

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