Results 111 to 120 of about 9,152,684 (304)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

open access: yesFEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj   +3 more
wiley   +1 more source

Lobbying in the EU: prospects and challenges of the mandatory transparency register

open access: yesFrontiers in Political Science
Lobbying practice is an essential part of the decision-making process in the European Union. The promotion of the practice of lobbying in institutions of the European Union quite naturally raised the question of regulating this activity according to the ...
Joan Ridao Martín   +1 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

"Governance through Policy Transfer in the External Relations of the European Union; The Case of Mercosur" [PDF]

open access: yes, 2006
The EU is at the forefront of an emerging pattern in international relations, so-called biregionalism. The Union increasingly seeks to establish relations with other world regions while actively promoting the emergence of regional integration projects ...
Lenz, Tobias
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Rational Choice and EU Politics [PDF]

open access: yes, 2007
[From the introduction]. Rational choice approaches to politics did not originate in the study of the European Union (EU), nor is ‘rational choice’ as such a theory of European integration or of EU politics.1 Rational choice, like constructivism, should ...
Pollack, Mark A.
core  

Unity in Diversity. The Language Policy of the European Union

open access: yesStudies in Logic, Grammar and Rhetoric, 2014
This paper explores the connections between the law and language in the European Union. The paper concerns the language policy of the European Union (EU) and English language priority. The EU faces challenges of finding a common ground for respecting the
doaj   +1 more source

THE DECIMATION OF THE INTRA EU BITS [PDF]

open access: yesChallenges of the Knowledge Society, 2018
The article concentrates on the process which led to the decimation of the intra-EU Bilateral Investment Treaties, due the affirmation of the public policy of European Union which takes precedence over the international obligations arisen for states ...
Beatrice Onica JARKA
doaj  

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

open access: yesFEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

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