Results 141 to 150 of about 777,754 (321)

Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels

open access: yesAdvanced Biology, EarlyView.
Bifunctional microgels carrying a linear DNA template and Ni2+‐activated NTA moieties are used as platform for cell‐free protein synthesis and in situ protein immobilization. By varying the concentration of NTA moieties in the microgels, the amount of GFP‐His immobilized inside the microgel and released to the microgel environment can be regulated ...
Anika Kaufmann   +2 more
wiley   +1 more source

Foundation for the ΔSCF Approach in Density Functional Theory [PDF]

open access: yesarXiv
We extend ground-state density-functional theory to excited states and provide the theoretical formulation for the widely used $\Delta SCF$ method for calculating excited-state energies and densities. As the electron density alone is insufficient to characterize excited states, we formulate excited-state theory using the defining variables of a ...
arxiv  

Scaling Up Synthetic Cell Production Using Robotics and Machine Learning Toward Therapeutic Applications

open access: yesAdvanced Biology, EarlyView.
Synthetic cells (SCs) hold great promise for biomedical applications, but manual production limits scalability. This study presents an automated method for large‐scale SC synthesis, integrating robotic liquid handling and machine learning‐driven high‐throughput characterization.
Noga Sharf‐Pauker   +7 more
wiley   +1 more source

Toward Design Principles for Biomolecular Condensates for Metabolic Pathways

open access: yesAdvanced Biology, EarlyView.
Biomolecular condensates are membrane‐less compartments found through‐out nature which can serve as reaction crucibles for biochemical processes. This review explores the design strategies underlying how condensates can be used in biotechnology to enhance multistep enzyme cascades including enhancement by mass action and substrate channeling, and ...
Alain A.M. André   +3 more
wiley   +1 more source

The Regulation of Trace Metal Elements in Cancer Ferroptosis

open access: yesAdvanced Biology, EarlyView.
The induction of ferroptosis inhibits tumor growth, enhances anticancer efficacy, and overcomes drug resistance. Recent evidence shows nonferrous metal elements play a role in ferroptosis. This review focuses on how trace metals regulate ferroptosis processes like iron accumulation, lipid peroxidation, and antioxidant defense.
Xiaoyan Wang   +5 more
wiley   +1 more source

A Novel Digitalization Approach for Smart Materials – Ontology‐Based Access to Data and Models

open access: yesAdvanced Engineering Materials, EarlyView.
In order to access heterogeneous material data and model‐based knowledge, the established ontology‐based data access (OBDA) is extended to include material models. This novel ontology‐based data and model access (OBDMA) enables the computation of new responses beyond stored data.
Jürgen Maas   +15 more
wiley   +1 more source

LASER INITIATION OF PETN: SELECTIVE PHOTOINITIATION REGIME

open access: yesВестник Кемеровского государственного университета, 2013
We describe and analyze the selective (resonance) laser-induced initiation of chemical decomposition reactions in PETN and propose a potential mechanism of the phenomenon.
E. D. Aluker   +2 more
doaj  

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