Results 191 to 200 of about 9,547 (313)

Aerodynamic design of supersonic experimental airplane

open access: yesAerodynamic design of supersonic experimental airplane
航空宇宙技術研究所 17-19 Jan. 2000 東京 日本 National Aerospace Laboratory 17-19 Jan. 2000 Tokyo Japan 超音速輸送機に対するNALの実験(NEXST(小型超音速実験機計画)-1)の第1段階において、超音速航行時での抵抗軽減のための空力設計に焦点が当てられた。CFD(計算流体力学)が、この研究には大変有益であると考えられた。設計段階で4つの空力概念が適用された。(1)矢羽根平面型、(2)ねじれ翼、(3)面積則胴体、(4)自然層流(NLF)翼である。NLFの概念は摩擦抵抗低減のために考案された。NLFは今までSSTに適用されたことはなく、このプロジェクト独自の試みである。NLFを実現するために、先ず ...
openaire  

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

open access: yesFEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow   +12 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Heterotropic regulation and negative homotropic cooperativity

open access: yesFEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea   +5 more
wiley   +1 more source

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