Results 251 to 260 of about 751,176 (305)

Characterizing a stable five-species microbial community for use in experimental evolution and ecology. [PDF]

Microbiology (Reading)
Castledine M, Pennycook J, Newbury A, Lear L, Erdos Z, Lewis R, Kay S, Sanders D, Sünderhauf D, Buckling A, Hesse E, Padfield D.   +11 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Correlated responses to selection across diverse environments during experimental evolution of Tetrahymena thermophila. [PDF]

Ecol Evol
Tarkington J, Zufall RA.
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Experimental evolution of hybrid populations to identify Dobzhansky-Muller incompatibility loci. [PDF]

Ecol Evol
Szabo N, Cutter AD.
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Adaptive radiation during long-term experimental evolution of the multicellular bacterium, Streptomyces

Munnoch JT, Vahtokari S, Kerr L, Hoskisson PA.   +3 more
europepmc   +1 more source

Experimental evolution of multicellularity via cuboidal cell packing in fission yeast. [PDF]

Evol Lett
Pineau RM, Kahn PC, Lac DT, Belpaire TER, Denning MG, Wong W, Ratcliff WC, Bozdag GO.   +7 more
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Experimental evolution suggests rapid assembly of the 'selfing syndrome' from standing variation in Mimulus guttatus. [PDF]

Front Plant Sci
Tusuubira SK, Kelly JK.
europepmc   +1 more source