Results 21 to 30 of about 6,873,841 (248)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

A Parametric Evaluation of Leakages in Water Distribution Networks

Engineering Proceedings
One of the main problems of water distribution systems is the management and the evaluation of water losses. At the Laboratory of Hydraulics and Maritime Constructions at the Università Politecnica delle Marche, experimental research on this topic was ...
Giovanna Darvini, Martina Gambadori, Luciano Soldini   +2 more
doaj   +1 more source

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source

Non-Invasive Estimation of Arterial Stiffness Using Photoplethysmography Sensors: An In Vitro Approach

Sensors
With advancing age, blood vessels undergo deterioration that causes structural and functional changes, including a progressive increase in arterial wall stiffness.
Gianluca Diana, Francesco Scardulla, Silvia Puleo, Salvatore Pasta, Leonardo D’Acquisto   +4 more
doaj   +1 more source

An Experimental Study of a Pico Hydro Turbine

Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 2022
Today, the storage and transporting of electricity from one place to another is still an unsolved problem. Besides, it is not economical to install power lines everywhere for low-voltage electronic circuits such as valves which are used to reduce leakage
Ufuk Durmaz, Ümit Pekparlak, Mehmet Berkant Özel, Orhan Yalçınkaya, Ümit Beyazgül   +4 more
doaj   +1 more source

Translophagy—A potential link between autophagy impairment and translational errors

FEBS Letters, EarlyView.
Neurodegenerative diseases are characterised by the accumulation of abnormal proteins and protein aggregates, but their origin often remains unknown. We propose that selective autophagy removes damaged protein‐making machinery, preventing errors during protein synthesis.
Mykola V. Korolchuk, Gailing Ma, Robin Antrobus, Diego Manni, Marco Trevisan‐Herraz, Mustafa Khwaja, Max Brown, Gavin D. Richardson, Masaaki Komatsu, Daniel Erskine, Viktor I. Korolchuk, Tetsushi Kataura   +11 more
wiley   +1 more source

Experimental and Computational Studies of a Prototype Combustion Chamber for a Power Steam Boiler with a Vertical Precessing Vortex [PDF]

Problems of the Regional Energetics
Increasing the efficiency of using fossil fuels at thermal power plants is an urgent task. This problem arises most acutely during the operation of pulverized coal power boilers using flare fuel combustion technologies.
Boiko E.A., Semenov I.I., Lebedev K.V., Kulichkin A.P.   +3 more
doaj   +1 more source

Measuring Setup for Experimental Research of Two-Coordinate Fiber-Optic Acceleration Sensors with Cylindrical Lenses

Sensors, 2022
The article presents the possibilities of using fiber-optic acceleration (FOC) sensors on products of rocket-space and aviation technology as part of information-measuring systems.
Elena Badeeva, Tatiana Murashkina, Andrey Motin, Saygid Uvaysov, Ainur Kozbakova, Daniel Sawicki   +5 more
doaj   +1 more source