Results 121 to 130 of about 892,836 (268)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

A Systematic Review and Expert Evaluation of Perioperative SGLT2 Inhibitor-Associated Ketoacidosis Case Reports. [PDF]

Acta Anaesthesiol Scand
Snel LIP, Li X, Jamaludin F, Siegelaar SE, Holleman F, Vriesendorp TM, DeVries JH, Hoekstra JBL, Preckel B, van Raalte DH, Hermanides J, Hulst AH.   +11 more
europepmc   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Validation of a Clinical Decision-Support Algorithm for Chronic Wound Classification and Treatment: An Expert Consensus. [PDF]

Int Wound J
Marques R, Pais-Vieira C, Lopes M, Neves-Amado J, Alves P.   +4 more
europepmc   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Development of a Cardiac Catheterization Laboratory Nursing Program Targeting Patients Undergoing Percutaneous Coronary Intervention for Acute Myocardial Infarction: A Delphi Study. [PDF]

J Multidiscip Healthc
Shan F, Xie N, Peng Y, Yang S, Zhou Y, Wang X, Zhou S, Huang P, Yang X.   +8 more
europepmc   +1 more source

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source

Diagnostic performance of biparametric versus multiparametric MRI for prostate cancer: a noninferiority, confirmatory observer study. [PDF]

Front Oncol
Fang X, Li J, Lv W, Liu L, Feng J, Liu L, Pan F, Zhang Y.   +7 more
europepmc   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Digital Competence in Managing Digital Resources Among Health Professions Faculty in Ethiopian Public Universities: Implications for CME/CPD Digital Transformation. [PDF]

J CME
Equlinet Misganaw A, Giletew Wondie A.
europepmc   +1 more source