Results 211 to 220 of about 26,057,468 (299)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

An exploratory analysis of the effects of human FYN and its Drosophila ortholog in Drosophila tissues. [PDF]

Sci Rep
McCabe M, Dunkel M, Negahdar A, Yoshinaga-Sakurai K, Jia D.   +4 more
europepmc   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

FEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu, Lanlan Li, Wenjuan Jia, Hanzhong Feng, Xiaoquan Yu, Youli Zhao, Fengzhi Yuan, Hui Ding, Junqiang Tian, Yong‐Xing He, Zhiping Wang   +10 more
wiley   +1 more source

Interplay between colorectal cancer-related lifestyles and the gut microbiome: an exploratory analysis of metagenomic data. [PDF]

Cancer Causes Control
Tammi R, Maukonen M, Kaartinen NE, Koponen K, Niiranen T, Méric G, Albanes D, Eriksson JG, Jousilahti P, Koskinen S, Pajari AM, Knight R, Havulinna AS, Salomaa V, Männistö S.   +14 more
europepmc   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

The P0.1 maneuver as an alternative method for assessing the validity of esophageal pressure measurements during assisted ventilation: an exploratory analysis. [PDF]

Intensive Care Med Exp
Bastian TM, Eimer C, Behmüller F, Weiler N, Bellani G, Schädler D, Becher T.   +6 more
europepmc   +1 more source

Digital twins to accelerate target identification and drug development for immune‐mediated disorders

FEBS Open Bio, EarlyView.
Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley   +1 more source

Physiological Characterisation of Severe Heart Failure Using Long-Duration Ambulatory ECG: A Retrospective Exploratory Analysis. [PDF]

Cureus
Khan JA, Ali U, Ahsan MT, Roy RC, Alamu OS, Alessi Longa F.   +5 more
europepmc   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Exploratory analysis of machine learning models for state and trait anxiety based on Spielberger questionnaire data in nursing students. [PDF]

BMC Med Educ
Salehinia R, Salehian S, Sangari MN, Sangari MAN, Abbassian H.   +4 more
europepmc   +1 more source