Results 191 to 200 of about 518,175 (290)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Extended-release buprenorphine treatment for opioid use disorder: A mixed-methods study of response and experience. [PDF]

Addiction
Lowry N, McKechnie A, Day E, Gilvarry E, Cowden F, Evans R, Locke R, Murray R, Vanderwaal R, Johnstone S, Hoare Z, Kelleher M, Mitcheson L, Marsden J.   +13 more
europepmc   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

An introduction to BASIC Guide: human biomonitoring and surveillance of chemical exposure in occupational settings. [PDF]

Ann Work Expo Health
Zare Jeddi M, Jones K, Leese E, Fustinoni S, Galea KS, Santonen T, Porras SP, Hopf NB, Göen T, Bader M, Tranfo G, Tristram A, Iavicoli I, Leso V, Koch HM, Pasanen-Kase R, Boogaard PJ, Persoons R, Esteban-López M, Verpaele S, Kasiotis KM, Machera K, Carrieri M, Palmen N, Duca RC, van Nieuwenhuyse A, Gonzales M, Haynes EN, Viegas S, Bessems J, Makris KC, Connolly A, Teixeira JP, Chung MK, Parsons PJ, Kumar E, Lin EZ, Kil J, Kwon JH, Tavares AM, Vekic AM, Souza G, Scheepers PTJ.   +42 more
europepmc   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Research training in occupational health and hygiene-design and pilot study. [PDF]

Ann Work Expo Health
Pisaniello D, Tejamaya M, Kadir A, Li Y, Tefera Y, Gaskin S.   +5 more
europepmc   +1 more source

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

FEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold, Janik Riese, Lancelot Seillier, Mark Kühnel, Julia Pannhausen, Charlotte O. J. Fröhlich, Christian Martin, Peter Boor, Matthias Saar, Danny D. Jonigk, Nadine T. Gaisa, Michael Rose   +11 more
wiley   +1 more source

A retrospective comparison of [¹⁸F]FDG radiation dose following a transition from conventional to long axial field of view PET/CT. [PDF]

Phys Eng Sci Med
Chen WJ, Rae WID, Kench PL, Willowson KP, Bailey DL, Bailey EA, Fearnside H, Kelliher E, Meikle SR.   +8 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Plant-derived extracellular vesicles in skin and bone tissue engineering: current status, challenges, and future perspectives. [PDF]

Front Bioeng Biotechnol
Lu C, Li D, Gao W, Sun X, Zhang Y, Yu Y, Fei C, Zhou P, Wu M.   +8 more
europepmc   +1 more source