Results 51 to 60 of about 246,522 (205)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Gaseous hydrocarbons absorption by ILs: insights from artificial intelligence strategy

open access: yesDiscover Applied Sciences
Accurate prediction of hydrocarbon solubility in ionic liquids (ILs) is essential for optimizing solvent selection and designing efficient chemical processes in industries such as energy production and environmental remediation.
Reyhaneh Alborz   +2 more
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Modeling form factor of Fagus orientalis trees using nonlinear regressions in the Caspian forests of Guilan Province [PDF]

open access: yesمجله جنگل ایران, 2019
One of the most important factors for determining the volume of trees is selection of an appropriate form factor. This study determined the most appropriate form factor formula and modeling the actual form factor in the Caspian region of northern Iran ...
Farivar Naghavi   +3 more
doaj  

Exponential smoothing for intermittent demand with demand basis updated more frequently than seasonality factors

open access: yesGestão & Produção, 2019
This study aims to present an alternative technique of exponential smoothing to estimate the demand for items with intermittent and seasonal demand. The usual technique would aggregate demand periods (months in quarters, for instance) to calculate a ...
Jorge Luiz de Biazzi
doaj   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

open access: yesFEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada   +5 more
wiley   +1 more source

Study on Dynamic Factors of Damped Beam Members Under Exponential Attenuation Loading

open access: yesBuildings
Blast loading is characterized as an impact loading with exponential attenuation, while damping serves as an intrinsic parameter that affects the vibration effect of beam members. The linear attenuation for simplified blast loading and undamped situation
Xin Hong, Shaobo Geng, Rui Feng
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

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