Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Evaluation of a Chemically Defined, Long-Term Extender for Liquid Storage of Stallion Semen. [PDF]
Brito LFC +5 more
europepmc +1 more source
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel +8 more
wiley +1 more source
Rabbit serum albumin as a novel biochemical modulator for enhancing female offspring production in commercial pig breeding through artificial insemination. [PDF]
Yodrug T, Hayakijkosol O, Wongtawan T.
europepmc +1 more source
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley +1 more source
Increasing the fertility of Sapudi sheep semen at 5°C storage temperature with the addition of oxytocin in a diluent of skim milk, egg yolk, and citrate. [PDF]
Mulyati S +9 more
europepmc +1 more source
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa +2 more
wiley +1 more source
Methyl Gallate Enhances Post-Thaw Boar Sperm Quality by Alleviating Oxidative Stress and Preserving Mitochondrial Function. [PDF]
Bu Y +9 more
europepmc +1 more source
Succinylation regulates boar sperm linear motility via reprogramming glucose metabolism. [PDF]
Wang Q +6 more
europepmc +1 more source
Moringa oleifera Lam. As a Herbal Antioxidant-Enriched Semen Extender in Cryopreservation: A Critical Review of Its Protective Potential. [PDF]
Firouzabadi AM +2 more
europepmc +1 more source

