Results 91 to 100 of about 6,735,254 (244)

Enhanced Förster Resonance Energy Transfer (FRET) on a Single Metal Particle

, 2016
We examined the effect of a metallic silver particle on Förster resonance energy transfer (FRET) between a nearby donor−acceptor pair. A donor-labeled oligonucleotide was chemically bound to a single silver particle, and then an acceptor-labeled ...
Joseph R. Lakowicz (24739), Yi Fu (315254), Jian Zhang (1682)   +2 more
core   +1 more source

Membrane‐Active Peptide Protects Against Inflammation by Targeting NLRP3 Activation at the Trans‐Golgi Network

Advanced Science, EarlyView.
The membrane‐active peptide Pep19‐2.5 reduces harmful inflammation by blocking activation of the NLRP3 inflammasome at trans‐Golgi network membranes. By targeting key membrane interactions, Pep19‐2.5 suppresses inflammatory IL‐1β production and alleviates allergic airway inflammation in mice, leading to reduced immune cell infiltration and improved ...
Jonas Engelhardt, Nico Kirsch, Aileen Kerfin, Lars P. Lunding, Dominic Ferber, Hannes Buthmann, Ilka Schreier, Carlotta Bosio, Ann‐Kathrin Dobbelstein, Anna Klawonn, Rebecca C. Coll, Lena Bauernhofer, Sandro Keller, Matthias Geyer, Michael Wegmann, Andra B. Schromm, Günther Weindl   +16 more
wiley   +1 more source

Kinetic Subpopulations Detected by Single-molecule Spectroscopy: Fundamental Property of Functional Nucleic Acids or Experimental Artefact?

CHIMIA, 2013
Single-molecule spectroscopy allows the direct observation of conformational dynamics in individual biomolecules. Here, we describe how single-molecule Förster resonance energy transfer (smFRET) reveals heterogeneous kinetics in the EBS1*/IBS1 ...
Sebastian L. B. König, Danny Kowerko, Roland K. O. Sigel   +2 more
doaj   +1 more source

A Universal Protein Ladder for Standardization of Diverse FRET Assays

Advanced Science, EarlyView.
Translating FRET measurements from in vitro to intracellular environments requires universal benchmarks. We present a modular protein ladder using TPR motifs to harmonize data across diverse platforms. This predictable calibration curve bridges single molecule FRET measurements, with cell based measurements including FLIM‐FRET.
Evelyn R. Smith, Katie L. Gelder, Lewis Hunter‐Craig, Daniel A. Bose, Timothy D. Craggs, Alison E. Twelvetrees   +5 more
wiley   +1 more source

Energy transfer leaves fingerprints in cyanine photoswitching behavior.

PLoS Computational Biology
Super-resolution microscopy resolves molecular structures in biological systems but is limited by properties of the fluorescent labels. dSTORM experiments with multiple Cy5 fluorophores in sub-10 nm spaced labelling positions have shown distinct ...
Vincent Ebert, Markus Sauer, Sören Doose   +2 more
doaj   +1 more source

A Small‐Molecule DEPTAC Rescues Cognitive Deficits by Targeted Dephosphorylation of Pathological Tau

Advanced Science, EarlyView.
TP2 is a fully synthetic small‐molecule dephosphorylation‐targeting chimera that recruits endogenous PP2A‐Bα to Tau, enabling targeted removal of pathological phosphate modifications. In two tauopathy mouse models, systemic TP2 reduces Tau pathology, preserves neuronal and synaptic integrity, improves neuroimmune homeostasis, and rescues cognition ...
Fei Sun, Yang Liu, Qiuzhi Zhou, Qingyuan Hu, Weixia Wang, Yue Xiao, Jingfen Su, Guanting Liu, Miaomiao Sun, Lixia Chen, Hua Li, Ying Yang, Jian‐Zhi Wang   +12 more
wiley   +1 more source

Alternating Shear Force of Respiration Regulates Cell Interactions of Fibroblasts and Macrophages to Promote Soft Tissue Integration of Chest Wall Polyetheretherketone Implants

Advanced Science, EarlyView.
Given that PEEK chest wall implants induce shear force at the soft tissue‐implant interface due to respiratory motion in the early in vivo post‐implantation stage. We confirmed that this mechanical stimulus triggers the PIEZO1/ITGB1/YAP1 signalling axis in L929 and the STAT6/p‐STAT6 pathway in RAW264.7, which in turn mediates adhesion and migration of ...
Rou Huang, Yangfan Huo, Jin Liu, Sida Liu, Minghai Ma, Hao Guo, Yuanquan Zhang, Xing Li, Xiao Liang, Jiawei Xiu, Ying Sun, Lijun Huang, Xiaolong Yan, Weiwei Wu, Lei Wang   +14 more
wiley   +1 more source

Bioinspired study of energy and electron transfer in photovoltaic system

Journal of Experimental Nanoscience, 2017
This study focuses on understanding the fundamentals of energy transfer and electron transport in photovoltaic devices with uniquely designed nanostructures by analysing energy transfer in purple photosynthetic bacteria using dye-sensitised solar cell ...
Md Moniruddin, Baurzhan Ilyassov, Evgeniya Seliverstova, Yerkin Shabdan, Nurlan Bakranov, Niyazbek Ibrayev, Nurxat Nuraje   +6 more
doaj   +1 more source

Assessment of Gate Width Size on Lifetime-Based Förster Resonance Energy Transfer Parameter Estimation

Photonics, 2015
Förster Resonance Energy Transfer (FRET) enables the observation of interactions at the nanoscale level through the use of fluorescence optical imaging techniques.
Sez-Jade Chen, Nattawut Sinsuebphon, Xavier Intes   +2 more
doaj   +1 more source

Photonic effects on the Förster resonance energy transfer efficiency [PDF]

, 2014
Förster resonance energy transfer (ET) between luminescent species is applied in bio-imaging, lighting and photovoltaics, and has an important role in photosynthesis.
Senden, Tim, Debye Institute, Rabouw, Freddy T., Den Hartog, Stephan A., Condensed Matter and Interfaces, Meijerink, A, Sub Condensed Matter and Interfaces   +6 more
core   +1 more source