Systematic evaluation and optimization of TaqMan qPCR assays targeting F57, ISMAP02, and IS900 for multiplex detection of Mycobacterium avium subsp. paratuberculosis [PDF]
Journal of Clinical MicrobiologyThis study aimed to develop a multiplex quantitative PCR (qPCR) assay for the detection of Mycobacterium avium subsp. paratuberculosis (MAP), the etiological agent of paratuberculosis disease, a chronic and endemic infectious disease affecting ruminant ...
Nathalie Bissonnette +5 more
doaj +2 more sources
Strain diversity in Mycobacterium avium subsp. paratuberculosis-positive bovine fecal samples collected in Switzerland [PDF]
Frontiers in Veterinary Science, 2023 Paratuberculosis or Johne’s disease is a chronic intestinal disease in domestic and wild ruminants. It affects global dairy economy and is caused by Mycobacterium avium subsp. paratuberculosis (MAP).
Melina Rasper-Hössinger +5 more
doaj +2 more sources
<i>Palisada yatsenii</i> sp. nov. (Ceramiales, Rhodophyta), a New Prostrate Red Alga From Mangroves of Guangxi, China. [PDF]
Ecol EvolA new red algal species, Palisada yatsenii sp. nov., is described from the high intertidal mangrove habitat in Qinzhou, Guangxi, China, representing the first record of Palisada in this unique niche. Second, integrative taxonomic analyses (morphology + rbcL/cox1 phylogenetics) confirm its independence, with distinct diagnostic traits (pale yellow ...
Zeng Z +7 more
europepmc +2 more sources
Use of F57 bags by the semi-automatic in vitro technique of gas production
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2014 Os cadinhos filtrantes (porosidade 1) originalmente usados na técnica in vitro semi-automática de produção de gases estabelecida no Laboratório de Produção de Gases da Escola de Veterinária da UFMG podem ocasionar interferências nos estudos de ...
Lúcio Carlos Gonçalves +2 more
exaly +3 more sources
Unlocking the Nutritional Potential of Gamma Amino Butyric Acid (GABA) Rice: Towards a Sustainable Approach to Metabolic Disorder Remedies. [PDF]
Food Sci NutrThis study addresses the critical issue of nutrient deficiencies associated with the widespread consumption of conventional milled rice, which often lacks essential vitamins and minerals due to the removal of the bran layer during processing. It demonstrated that controlled germination of paddy rice, followed by parboiling, substantially increases the ...
Onyeka UE +5 more
europepmc +2 more sources
Enhancing the Protein Stability of an Anticancer VHH‐Fc Heavy Chain Antibody through Computational Modeling and Variant Design [PDF]
Advanced ScienceVHHs (also known as nanobodies) are important therapeutic antibodies. To prolong their half‐life in bloodstream, VHHs are usually fused to the Fc fragment of full‐length antibodies.
Yuan Fang +10 more
doaj +2 more sources
Aggressive Juvenile Trabecular Ossifying Fibroma in a 4-Year-Old: Case Report Bringing Diagnostic Challenges and Surgical Management. [PDF]
Case Rep DentJuvenile trabecular ossifying fibroma (JTOF) is an uncommon benign fibro‐osseous neoplasm of the craniofacial skeleton that predominantly affects children and may clinically and radiographically resemble other aggressive lesions. We describe the case of a 4‐year‐old boy with a rapidly enlarging, painless maxillary mass.
Spenrath B +4 more
europepmc +2 more sources
Ligninolytic Enzyme Content of Mushroom <i>Pleurotus ostreatus</i> and <i>Agaricus bisporus</i> Extracts and In Vitro Rumen Fermentation of Fungal Extract-Treated Wheat Straw. [PDF]
Food Sci NutrMushrooms produce enzymes via the radical apparatus to attack lignin and cellulose and we evaluated extracts from byproducts of Pleurotus ostreatus and Agaricus bisporus in terms of content of ligninolytic enzymes and as sustainable means of enhancing the degradability of forages. The extracts contained ligninolytic enzymes and were used to treat wheat
Daniso E, Spanghero M.
europepmc +2 more sources
Effect of exogenous glucoamylase on ruminal in situ and in vitro dry matter and starch degradability of cereal grains in beef cattle [PDF]
Animal BioscienceObjective To evaluate the effects of glucoamylase on ruminal in situ and in vitro dry matter degradability (IVDMD), in vitro starch degradability (IVSD), volatile fatty acids (VFA), gas production and methane production using cereal grains as substrates ...
Lin Mu +8 more
doaj +2 more sources
Detection of Mycobacterium avium Sub sp. paratuberculosis in Pasteurized Milk Samples in Northeast of Iran by Culture, Direct Nested PCR and PCR Methods [PDF]
Iranian Journal of Chemistry & Chemical Engineering, 2020 Mycobacterium avium Subsp. paratuberculosis (MAP) is a gram-positive, small, acid-fast bacillus with high environmental resistance. In animals, especially ruminants, it leads to Paratuberculosis (PTB) or Johne's disease, which is chronic granulomatous ...
Nasim Sadeghi +2 more
doaj +1 more source