Results 151 to 160 of about 131,711 (285)

Investigating the Cytoskeleton of DRGs Using Cryo‐Electron Microscopy and Deep Learning

Cytoskeleton, EarlyView.
ABSTRACT We describe a method for determining the ultrastructural organization of axons and varicosities of cultured dorsal root ganglion (DRG) neurons using cryogenic electron microscopy (cryo‐EM). Cryo‐EM reveals the dimensions, proximity, and overall organization of biological specimens in a near‐native state, avoiding artifacts of fixation and ...
M. Neal Waxham, Mihir Relan, Matthew T. Swulius, Andrea K. H. Stavoe   +3 more
wiley   +1 more source

Ultra-rapid cryo-EM data acquisition method enabled by continuous recording based beam image shift. [PDF]

Nat Commun
Yang Q, Huang X, Wu C, Zeng Y, Zhang X.
europepmc   +1 more source

High‐Dimensional Spectral Flow Cytometry Panel for Immunophenotypic Characterization of Fixed Whole Blood Samples

Cytometry Part A, EarlyView.
ABSTRACT Peripheral blood mononuclear cells (PBMCs) are commonly used for immune profiling in clinical research, but their isolation and cryopreservation present logistical challenges, particularly in settings with limited access to research facilities. To address this, we implemented a whole blood fixation protocol to preserve surface protein epitopes
Vaishnavi Parthasarathy, Alexander T. Heubeck, Tyanna Stuckey, Veronica Hernandez, Blessing Musgrove, Maia M. Bennett‐Boehm, Troy Torgerson, Peter Skene, Julian Reading   +8 more
wiley   +1 more source

The Global Airways Unit: A Multidisciplinary Strategy for Early Diagnosis and Comorbidity Detection. [PDF]

World J Otorhinolaryngol Head Neck Surg
Conti DM, Correa EJ, Del Cuvillo A, Abdullatif JD, Alvarez-Gutiérrez FJ, Contreras RC, Cimbollek S, Millán RC, Fernández AG, Pérez-Chada D, Pérez-Grimaldi F, Rabino AE, Romero-Falcón MA, Soria M, Soto-Campos JG, Spuches RB, Maza-Solano J.   +16 more
europepmc   +1 more source

Fluorescence Behavior of Water‐Soluble Porphyrins Immobilized on LTA Zeolite and Its Chlorogenic Acid Polyphenol Sensing in Coffee Wastewater

ENERGY &ENVIRONMENTAL MATERIALS, EarlyView.
During coffee bean and cherry washing, contaminants are released into water, making this process a major source of water pollution. Chlorogenic acid can be efficiently detected using hybrid zeolite–porphyrin materials by measuring fluorescence quenching, achieving detection limits lower than current regulatory thresholds.
João Otávio Donizette Malafatti, Gabriele Magna, Francesco Pizzoli, Fabrizio Caroleo, Cadia D'Ottavi, Williane da Silva Freitas, Ailton José Moreira, Elaine Cristina Paris, Luiz Henrique Capparelli Mattoso, Corrado Di Natale, Roberto Paolesse   +10 more
wiley   +1 more source

Protocol for short-term in vitro propagation of patient-derived group 4 medulloblastoma MBT375 tumor cells. [PDF]

STAR Protoc
Custers S, Venugopal C, Singh SK.
europepmc   +1 more source

Developmental pathways to autism in tuberous sclerosis complex: Evidence from a longitudinal cohort

Epilepsia, EarlyView.
Abstract The association between autism spectrum disorder (hereafter referred to as autism) and tuberous sclerosis complex (TSC) is well established, yet the developmental pathways linking genetic mutation, cortical pathology, and epilepsy with autism remain unclear. The Tuberous Sclerosis 2000 Study recruited children newly diagnosed with TSC (N = 125)
Fiona S. McEwen, Abigail K. Runicles, Holan Liang, Emma Woodhouse, Lisa Underwood, Elizabeth Shephard, Edward D. Barker, Fintan Sheerin, Juul W. Steenbruggen, Tuberous Sclerosis 2000 Study Group, John R. W. Yates, Charlotte Tye, Patrick F. Bolton   +12 more
wiley   +1 more source

Modelling habitat suitability and estimating protected areas coverage for Falco cherrug across its wintering habitats. [PDF]

Sci Rep
Yousefi M, Mohammadi S, Khani A, Adibi MA, Goudarzi F, Nezami B, Abtin E.   +6 more
europepmc   +1 more source

Febrile status epilepticus and epileptogenesis: The FEBSTAT study

Epilepsia Open, EarlyView.
Abstract The multicenter FEBSTAT study (Consequences of Prolonged Febrile Seizures in Childhood: https://grantome.com/grant/NIH/R37‐NS043209‐12; PI S. Shinnar) examined the outcome of febrile status epilepticus (FSE) in over 200 prospectively enrolled infants, with many followed for 10 years after FSE.
Darrell V. Lewis, Douglas R. Nordli Jr., Douglas R. Nordli III, Shlomo Shinnar, Stephen Chan, Jacqueline A. Bello, L. Matthew Frank, James Voyvodic, William Gallentine, Syndi Seinfeld, Yoshimi Sogawa, Erica Weiss, David Masur, Yuan Xu, Solomon L. Moshé   +14 more
wiley   +1 more source

Brief Report: Proposed Clinical Trial Guidelines Based on Clinicopathologic Heterogeneity of Metastatic Pulmonary Large Cell Neuroendocrine Carcinoma. [PDF]

J Thorac Oncol
Choudhury NJ, Gezelius E, Arfe A, Baine M, Falcon CJ, Yu H, Rekhtman N, Rudin CM.   +7 more
europepmc   +1 more source