Results 191 to 200 of about 121,696 (304)

From the sea to DNA barcode: Optimized preservation method for gelatinous zooplankton integrative taxonomy

Limnology and Oceanography: Methods, EarlyView.
Abstract Assigning taxonomic identity and thus assessing taxonomic diversity in gelatinous zooplankton is challenging due to their fragility and difficult preservation. Formalin fixation compromises DNA integrity, while flash‐freezing, ethanol, and acetone preservation affect morphological features. In this study, we developed a methodological protocol
Claudia Traboni, Rade Garić, Rita Marino, Paolo Sordino, Priscilla Licandro   +4 more
wiley   +1 more source

Exploratory Evaluation of Self-Reported Periodontitis Among Adult Population From Comitán Chiapas, Mexico. [PDF]

Clin Exp Dent Res
Falcón-Flores JA, Jiménez-Corona ME, Rangel-Nieto IG, Vazquez-Duran M, Jiménez-Corona A.   +4 more
europepmc   +1 more source

Polyglycerol‐Based Lipids: A Next‐Generation Alternative to PEG in Lipid Nanoparticles for Advanced Drug Delivery Systems

Macromolecular Rapid Communications, EarlyView.
Linear polyglycerol is proposed as a PEG‐alternative in mRNA‐loaded lipid nanoparticles to limit activation of anti‐PEG antibodies and reduce immunogenicity. Resulting nanoparticles may provide similar gene delivery capabilities, while preventing binding of anti‐PEG antibodies compared to PEGylated counterparts.
Yara Ensminger, Rashmi Rashmi, Michael Karimov, Gideon Nölte, Markus Hafke, Ann‐Cathrin Schmitt, David Díaz‐Oviedo, Johannes Köbberling, Rainer Haag   +8 more
wiley   +1 more source

Protocol for generating and characterizing Matrigel-free prostate cancer patient-derived organoids. [PDF]

STAR Protoc
Dolgos R, Parmentier R, Wang J, Templeton AJ, Mertz KD, Pueschel H, Seifert H, Mortezavi A, Vlajnic T, Rentsch CA, Bubendorf L, Le Magnen C.   +11 more
europepmc   +1 more source

Enrichment of Hyaluronic Acid Binding Tumor Cells by Modulation of Selective Adhesion on Microgel Surfaces

Macromolecular Rapid Communications, EarlyView.
We have developed a thermoresponsive microgel system for the selective enrichment of hyaluronic acid‐binding tumor cells. By modulating the adhesion on the microgel surface via temperature changes from 37°C to 30°C, we demonstrate a novel proof‐of‐principle for separating these tumor cell from white blood cells, which has potential applications in ...
Melanie Schmidt, Dilay Karayel, André Franken, Janita Müller, Laura Hartmann, Klaus Pantel, Tanja Fehm, Hans Neubauer, Stephan Schmidt   +8 more
wiley   +1 more source

A Tutorial for Isolating, Characterizing, and Inducing Presenescence in Human Periodontal Ligament and Dental Pulp Stem Cells. [PDF]

Curr Protoc
Leme KSV, Golim MA, Braz AMM, Deffune E, Salvadori DMF.   +4 more
europepmc   +1 more source

The toxin–antitoxin complex Fic‐1–AntF functions as a deAMPylase that regulates the activity of DNA gyrase

mLife, EarlyView.
Abstract Toxin–antitoxin (TA) systems found in diverse bacteria play important roles in their adaptation to changing environments. The toxin of the Fic‐1–AntF TA pair from Pseudomonas bijieensis strain 2P24 inhibits bacterial DNA replication by attacking the subunit B of DNA gyrase (GyrB) via AMPylation, while the antitoxin AntF blocks its enzymatic ...
Furong Chen, Liwei Guo, Canhua Lu, Wenjun Jiang, Zhao‐Qing Luo, Junfeng Liu, Li‐Qun Zhang   +6 more
wiley   +1 more source

Analgesic efficacy of meloxicam vs ibuprofen on pain after third molar surgery in adult patients. A randomized controlled clinical trial. [PDF]

Med Oral Patol Oral Cir Bucal
Falcón-Perez R, Pradenas-Loaiza V, Bertrán-Delgado C, Aguilera F, Kunstmann-Camino J.   +4 more
europepmc   +1 more source

Lectotypifications and taxonomic changes in the holoparasitic Orobanchaceae

Nordic Journal of Botany, EarlyView.
Based on further extensive studies of specimens in various herbaria, lectotypes are designated for many taxa of holoparasitic Orobanchaceae. In particular, 47 names in the genera Boschniakia (incl. Xylanche), Cistanche, Orobanche, Phelipanche and Phelypaea are lectotypified.
Holger Uhlich, Stefan Rätzel, Óscar Sánchez Pedraja, Renata Piwowarczyk, Valerio Lazzeri, Patrice Descombes   +5 more
wiley   +1 more source

Protocol for preparation and execution of collagen plug contraction assay to measure fibroblast contractility induced by growth factors. [PDF]

STAR Protoc
Wuppukondur SN, Patel JC, Coulson-Thomas VJ, Gesteira TF.   +3 more
europepmc   +1 more source