RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Open Proximal Rectus Femoris Tendon Full-Thickness Tear Repair With Double-Row Suture Anchor Transosseous-Equivalent Construct With Biologic Augmentation Using Bone Marrow Aspirate Concentrate and Bovine Bioinductive Collagen Implant. [PDF]
Maldonado DR, Bastidas A, Hovsepian JM.
europepmc +1 more source
Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj +3 more
wiley +1 more source
Transosseous Equivalent Meniscus Allograft Transplantation Using an All-Suture-Based Anchor Technique. [PDF]
Barney ME +5 more
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Privacy-Preserving and Poisoning-Robust Federated Learning for Industrial IoT. [PDF]
Yin H, Chen C, Zhang J, Yu D, Liu S.
europepmc +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Biobased Oligo- and Polyesters from Fatty Acids with Demonstrated Biodegradability or Recyclability for Industrial Applications in a Circular Economy. [PDF]
Gröger H.
europepmc +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Endoscopic closure of a perforated peptic ulcer using a detachable snare loop in an elderly patient at high risk for surgery. [PDF]
Eom SS, Kang S, Kim NH, Kim JW.
europepmc +1 more source

