Results 161 to 170 of about 5,956,341 (214)
KDAC6 has been associated with cell motility and actin structures, but specific domain contributions are not established. The two catalytic domains have differential effects on motility and F‐actin regulation, affecting cortical F‐actin density, motility, stress fibers, and cell spreading.
Taylor V. Joseph +7 more
wiley +1 more source
Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
FIT Soul Fashion Show: 1982, Dress Rehearsal part 2
Dress Rehearsal of The FIT Soul Club's presentation of the 12th annual Soul Fashion Show: "12 Years of Sophistication." Featuring work by student designers Kevin Pinnock, Sherrie Hobson, Gail Branch, Steve Cutting, Linda Fasion, Shanault Wadley, Keith ...
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Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
The FIT Soul Club presents the 1990 Soul Fashion Show: "The Best of Everything." Produced and Directed by Clara Branch. Co-director: Leland Michael. The fashion show features work by FIT student designers.NOTE: Portions of this video may be muted due to ...
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Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta +7 more
wiley +1 more source
100 Years of Fashion: Part Two
The Museum at FIT is excited to present 100 Years of Fashion, a two-part introduction to the history of fashion from the late 19th century through the 1990s. These two videos were originally created for high school students enrolled in FIT's Summer Live,
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Threonine 348 regulates the subcellular localization of PTEN
Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley +1 more source
Fashion in the Nineties Symposium: "1990s Fashion Ephemera: Trends, Desire, and Exclusivity"
Graphic designer Paul Boudens and assistant professor and director of the MA in Fashion Studies at The New School Parsons Paris Dr. Marco Pecorari discuss 90s Fashion Ephemera: Trends, Desire, and Exclusivity for The Museum at FIT's 26th academic ...
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