Results 21 to 30 of about 14,556 (244)

Early prosthetic joint infection due to Ureaplasma urealyticum: Benefit of 16S rRNA gene sequence analysis for diagnosis

open access: yesJournal of Microbiology, Immunology and Infection, 2019
Detection of the most frequently bacteria involved in prosthetic joint infection (PJI) is usually performed by conventional cultures. We report a case of early PJI due to Ureaplasma urealyticum, diagnosed by 16S rRNA gene sequence analysis, which ...
Caroline Rouard   +10 more
doaj   +1 more source

Comparing Culture and Multiplex PCR Methods to Examine Fastidious Bacteria in Otitis Externa and Media

open access: yesJournal of Pure and Applied Microbiology, 2017
Ear infection or otitis is a generic term used to refer to ear infection or inflammation. The purpose of the present study was to compare culture and Multiplex PCR methods to identify Alloiococcus otitidis, Moraxella catarrhalis, and Haemophilus ...
Tina Delsouz Bahri   +5 more
doaj   +1 more source

Detection of Fastidious Vaginal Bacteria in Women with HIV Infection and Bacterial Vaginosis [PDF]

open access: yesInfectious Diseases in Obstetrics and Gynecology, 2009
Background. Fastidious bacteria have been associated with bacterial vaginosis (BV) using PCR methods. We assessed the prevalence of these bacteria in HIV-1 infected women and their relationship with vaginal pH and shedding of HIV-1 RNA.Methods. 64 cervicovaginal lavage (CVL) samples were collected from 51 women.
Mitchell, Caroline   +7 more
openaire   +2 more sources

Genome Analysis of Haplotype D of Candidatus Liberibacter Solanacearum

open access: yesFrontiers in Microbiology, 2018
Candidatus Liberibacter solanacearum (Lso) haplotype D (LsoD) is a suspected bacterial pathogen, spread by the phloem-feeding psyllid Bactericera trigonica Hodkinson and found to infect carrot plants throughout the Mediterranean.
Leron Katsir   +9 more
doaj   +1 more source

Metagenomic next-generation sequencing in osteoarticular infections with a focus on pediatrics: current concepts and clinical applications

open access: yesEFORT Open Reviews, 2023
Osteoarticular infections (OAI) are a significant cause of morbidity and mortality. Cultures and serology are some of the gold standards for identifying infection but are often unable to provide a timely diagnosis or a diagnosis at all. Genetic testing
Marco Gupton, Jessica Burns
doaj   +1 more source

Reliability of quantitative real-time PCR for bacterial detection in cystic fibrosis airway specimens. [PDF]

open access: yesPLoS ONE, 2010
The cystic fibrosis (CF) airway microbiome is complex; polymicrobial infections are common, and the presence of fastidious bacteria including anaerobes make culture-based diagnosis challenging.
Edith T Zemanick   +5 more
doaj   +1 more source

Grapevines Pretreated with Biocontrol Bacteria Have an Altered Transcriptional Response to Xylella fastidiosa and a Reduction in Pierce's Disease Severity

open access: yesMolecular Plant-Microbe Interactions
In a previous DNA-based microbiome study of grapevines in areas of Pierce's disease (PD) pressure, we determined that taxa belonging to the Pseudomonas and Achromobacter genera negatively associated with PD severity and titer of the causal agent, Xylella
Elizabeth Deyett   +7 more
doaj   +1 more source

False Positive Cases in Automated Blood Culture Systems Due to Hyperleukocytosis

open access: yesAnnals of Clinical Microbiology, 2023
Automated blood culture systems are widely used in clinical microbiology laboratories to minimize the workload of laboratory personnel and permit fast turnaround times.
Do-Hoon Kim, Namhee Ryoo
doaj   +1 more source

A more rapid method for transformation of Helicobacter pylori

open access: yesmSphere
Helicobacter pylori is a major causative agent in several upper gastroduodenal tract diseases, including gastric cancer. The development of methods to genetically manipulate H.
Caroline D. Skene, Richard L. Ferrero
doaj   +1 more source

Effectiveness of nutrient media for the recovery of lyophilisates of fastidious microorganisms: Evidence from Streptococcus spp.

open access: yesUkrainian Journal of Veterinary Sciences, 2022
To restore the biological and morphological properties of fastidious bacteria during lyophilization, one of which is a type of streptococcus – bacteria of the genus Streptococcus, it is necessary to use expensive specialized nutrient media that are ...
Serhii Boianovskyi, Kateryna Rudnieva
doaj   +1 more source

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