Results 231 to 240 of about 133,640 (303)

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Flourishing chemosynthetic life at the greatest depths of hadal trenches. [PDF]

Nature
Peng X, Du M, Gebruk A, Liu S, Gao Z, Glud RN, Zhou P, Wang R, Rowden AA, Kamenev GM, Maiorova AS, Papineau D, Chen S, Gao J, Liu H, He Y, Alalykina IL, Dolmatov IY, Zhang H, Li X, Malyutina MV, Dasgupta S, Saulenko AA, Shilov VA, Liu S, Xie T, Qu Y, Song X, Zhang H, Liu H, Zhang W, Huang X, Xu H, Xu W, Mordukhovich VV, Adrianov AV.   +35 more
europepmc   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

On the genetic structure and origin of the little ground squirrel Spermophilus pygmaeus (Pallas, 1778) in the North Caucasus. [PDF]

Vavilovskii Zhurnal Genet Selektsii
Tembotova FA, Amshokova AK, Gudova MS.
europepmc   +1 more source

The Strud crustacean fauna (Late Devonian, Belgium): updated review and palaeoecology of an early continental ecosystem

, 2018
Eva Du Tien Hat, Nicolas Rabet, Pierre Gueriau   +2 more
openalex   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

Wildlife Dermatophytoses in Central Italy (Umbria and Marche Regions): A Fifteen-Year Investigation (2010-2024). [PDF]

J Fungi (Basel)
Crotti S, Cruciani D, D'Avino N, Fiorucci A, Morganti G, Paoloni D, Papini M, Piscioneri V, Ranucci A, Spina S, Gobbi M.   +10 more
europepmc   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

Rooting for function: community-level fine-root traits relate to many ecosystem functions. [PDF]

New Phytol
Barry KE, Hennecke J, Weigelt A, Bergmann J, Bruelheide H, Freschet GT, Iversen CM, Kuyper TW, Laughlin DC, McCormack ML, Roumet C, van der Plas F, van Ruijven J, Wijsmuller R, Auge H, Eisenhauer N, Haase J, Nock CA, Oelmann Y, Wilcke W, Mommer L.   +20 more
europepmc   +1 more source

Molecular determinants of signal transduction in tropomyosin receptor kinases

FEBS Open Bio, EarlyView.
Tropomyosin receptor kinases control critical neuronal functions, but how do the same receptors produce diverse cellular responses? This review explores the structural mechanisms behind Trk signaling diversity, focusing on allosteric modulation and ligand bias.
Giray Enkavi
wiley   +1 more source