Results 41 to 50 of about 318,728 (192)

Development of an HEK293 Suspension Cell Culture Medium, Transient Transfection Optimization Workflow, and Analytics for Batch rAAV Manufacturing

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Recombinant adeno associated virus (rAAV) vectors have become popular delivery vehicles for in vivo gene therapies, but demand for rAAVs continues to outpace supply. Platform processes for rAAV production are being developed by many manufacturers, and transient chemical transfection of human embryonic kidney 293 (HEK293) cells is currently the
Erica A. Green, Qiang Fu, Nelson Ndahiro, Thomas M. Leibiger, Yongdan Wang, Yongsuk Lee, Kelvin H. Lee, Michael Betenbaugh, Seongkyu Yoon, David J. McNally   +9 more
wiley   +1 more source

Flux Sampling Suggests Metabolic Signatures of High Antibody‐Producing CHO Cells

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Chinese hamster ovary (CHO) cells remain the industry standard for producing numerous therapeutic proteins, particularly monoclonal antibodies (mAbs). However, achieving higher recombinant protein titers remains an ongoing challenge and a fundamental understanding of the cellular mechanism driving improved bioprocess performance remains ...
Kate E. Meeson, Joanne Watson, Susan Rosser, Ellie Hawke, Andrew Pitt, Tessa Moses, Leon Pybus, Magnus Rattray, Alan J. Dickson, Jean‐Marc Schwartz   +9 more
wiley   +1 more source

Physiology and Robustness of Yeasts Exposed to Dynamic pH and Glucose Environments

Biotechnology and Bioengineering, EarlyView.
The authors investigated how three Saccharomyces cerevisiae strains respond to dynamic substrate and pH oscillations using dynamic microfluidic single‐cell cultivation (dMSCC) and intracellular biosensors. By combining performance and robustness analysis of selected intracellular parameters, growth and morphological analysis, the authors identified ...
Luca Torello Pianale, Luisa Blöbaum, Alexander Grünberger, Lisbeth Olsson   +3 more
wiley   +1 more source

Pleading Patents: Predicting the Outcome of Statutorily Heightening Pleading Standards [PDF]

, 2015
The tension between an extremely barebones Federal Rules of Civil Procedure Form 18 for patent infringement lawsuits and Supreme Court case law through Twombly and Iqbal has made it difficult for courts to dismiss frivolous patent litigation at the ...
Rangarajan, Arjun
core   +1 more source

Systematic Investigation of Impact of Antifoam and Extracellular Vesicles on Fouling of Hollow Fiber Filters in Intensified Perfusion Processes Highlights the Key Impact of Antifoam

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Ultra‐high cell density perfusion cultures are becoming increasingly attractive for biologics manufacturing due to their ability to boost productivity and offer a flexible manufacturing footprint. However, these intensified perfusion processes pose challenges, particularly concerning the performance of hollow fiber filters used as cell ...
Sri Ranganayaki Madabhushi, Qianying Zuo, Carol Zhou, Chelsea Phuangthong, Nathaniel Ruppert, Tiffany Tang, Francisco Pelaez   +6 more
wiley   +1 more source

Small Intestine on a Chip Demonstrates Physiologic Mucus Secretion in the Presence of Lacticaseibacillus rhamnosus Biofilm

Biotechnology and Bioengineering, EarlyView.
Dash and coworkers have fabricated a small intestine on a chip, which encapsulates the physiology and biofilm of the small intestinal epithelium. The dynamic system showed villus differentiation within 5 days of flow, and the mucus layer and biofilm showed small intestinal characteristics.
Sanat Kumar Dash, Cláudia N. H. Marques, Gretchen J. Mahler   +2 more
wiley   +1 more source

Patent Misjoinder [PDF]

, 2013
The Leahy-Smith America Invents Act effectively repealed aspects of the Federal Rules of Civil Procedure by creating a new statutory section governing joinder of accused infringers and consolidation of actions for trial in most patent infringement cases.
Taylor, David O.
core   +1 more source

Modeling the Migration and Growth of Shewanella Oneidensis MR‐1 in a Diffusion‐Dominated Microfluidic Gradient Chamber Under the Influence of an Antibiotic Concentration Gradient

Biotechnology and Bioengineering, EarlyView.
The authors investigate how Shewanella oneidensis navigates toxic environments to access nutrients in a simplified soil environment, combining modeling and experimental data. Their results reveal that motility, rather than chemotaxis, enables bacterial migration into antibiotic‐laden zones to reduce nitrate, suggesting a key mechanism for pollutant ...
Somayeh G. Esfahani, Reinaldo E. Alcalde, Albert J. Valocchi, Robert A. Sanford, Charles J. Werth   +4 more
wiley   +1 more source

Rethinking Civil Contempt Incarceration [PDF]

, 2006
Under current federal law civil contempt is governed by the Federal Rules of Civil Procedure, yet it often results in incarceration. This incarceration can, and in a few cases has been, indefinite.
Kornberg, Jessica C
core   +1 more source

A New Aerosol‐Based Photobioreactor for the Cultivation of Cyanobacteria on Luffa

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Cyanobacteria are promising organisms for sustainable biotechnology due to their ability to grow photoautotrophically and their wide range of products. Many cyanobacteria grow in the form of biofilms, which is why the development of photobioreactors (PBR) for the cultivation of cyanobacteria in the form of biofilms is of great interest ...
Jonas Kollmen, Andreas Stiefelmaier, Ramtin Mofrad, Dorina Strieth   +3 more
wiley   +1 more source