Results 171 to 180 of about 20,184 (214)
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Anthracycline antibiotic reduction by spinach ferredoxin-NADP+ reductase and ferredoxin
Biochemistry, 1985Spinach NADPH:ferredoxin oxidoreductase (EC 1.6.7.1) catalyzes the NADPH-dependent reduction of the anthracyclines daunomycin, aclacinomycin A, and nogalamycin and their respective 7-deoxyanthracyclinones. Under anaerobic conditions, the endogenous rate of O2 reduction by NADPH catalyzed by ferredoxin reductase (0.12 s-1 at pH 7.4) is augmented by the ...
J, Fisher, B R, Abdella, K E, McLane
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Inhibition of pea ferredoxin–NADP(H) reductase by Zn‐ferrocyanide
European Journal of Biochemistry, 2004Ferredoxin–NADP(H) reductases (FNRs) represent a prototype of enzymes involved in numerous metabolic pathways. We found that pea FNR ferricyanide diaphorase activity was inhibited by Zn2+ (Ki 1.57 µm). Dichlorophenolindophenol diaphorase activity was also inhibited by Zn2+ (Ki 1.80 µm), but the addition of ferrocyanide was required, indicating that the
Daniela L Catalano, Dupuy +2 more
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Proteolytic degradation of ferredoxin-NADP reductase during purification from spinach
Archives of Biochemistry and Biophysics, 1990Ferredoxin-NADP reductase (FNR) was rapidly isolated from spinach leaves with special care to suppress proteolytic degradation. The molecular mass of this FNR preparation was estimated to be 35 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Limited proteolysis of 35-kDa FNR to 33-kDa FNR was effectively suppressed by high pH (at pH 9.
M, Shin +5 more
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Interaction of ferredoxin-NADP+ reductase from Anabaena with its substrates
Archives of Biochemistry and Biophysics, 1991The interaction of ferredoxin-NADP+ reductase from the cyanobacterium Anabaena variabilis with its substrates, NADP+ and ferredoxin, has been studied by difference absorption spectroscopy. Several structural analogs of NADP+ have been shown to form complexes the stabilities of which are strongly dependent on the ionic strength of the medium.
J, Sancho, C, Gómez-Moreno
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Purification, properties, and cellular localization of Euglena ferredoxin-NADP reductase
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1987Ferredoxin-NADP reductase from Euglena gracilis Klebs var. Bacillaris Cori purified to apparent homogeneity, yields a typical 36 kDa and an unusual 15 kDa polypeptide on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, exhibits a typical flavoprotein spectrum, contains FAD, and catalyzes NADPH-dependent iodonitrotetrazolium-violet diaphorase,
A J, Spano, J A, Schiff
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[40] Ferredoxin-NADP reductase from spinach
1971Publisher Summary This chapter discusses the determination of ferredoxin–NADP reductase from spinach. Ferredoxin–NADP reductase is a chloroplast flavoprotein and catalyzes the reduction of NADP with reduced ferredoxin. The crystalline enzyme shows not only ferredoxin–NADP reductase activity, but also a number of other enzymatic activities, such as ...
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Complex formation by ferredoxin-NADP+ reductase with ferredoxin or NADP+
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1973Abstract Complex formation by ferredoxin-NADP + reductase (NADPH:ferredoxin oxidoreductase, EC 1.6.99.4) with ferredoxin was measured by the independent methods based on the changes of circular dichroism, fluorescence intensity and the chromatographic behavior on a Sephadex G-75 column of the two proteins after mixing.
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Enzymes of Plastid Ribosome-deficient Mutants. Ferredoxin-NADP+ Reductase
Biochemie und Physiologie der Pflanzen, 1981Summary Green and white leaves of the barley mutant line "albostrians" were investigated for the presence of soluble and thylakoid-bound ferredoxin-NADP + reductase. The mutant plastids of white leaves show a ribosome deficiency. White leaves contain appr.
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Structure and Function of Ferredoxin-NADP Reductase Complex
1990In 1963, Shin et al. (1) first isolated ferredoxin-NADP reductase (FNR, EC. 1.18.1.2) in crystalline form from spinach and demonstrated that the crystallized enzyme reduced NADP when it was added back together with ferredoxin to illuminated chloroplasts from which “built-in” FNR had been removed.
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[Influence of ferredoxin on ferredoxin-NADP reductase].
Planta, 2014Transhydrogenase and diaphorase activity of ferredoxin-NADP reductase are enhanced by plant ferredoxins. This stimulation is specific; ferredoxin cannot be replaced by sulfhydryl compounds such as cysteine or dithiothreitol, the apoprotein of ferredoxin or Fe(2+), Fe(3+) ions.The effect is particularly obvious with the reductase from the heterokont ...
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