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Specificity of ferulic acid (feruloyl) esterases
Biochemical Society Transactions, 1998Specificity of ferulic acid (feruloyl) esterases G. Williamson’, C. B. Faulds and P. A. Kroon Biochemistry Department, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, U.K. 205 moyl esters (manifest as a K,,, of between lop6 Feruloyl esterases are a subclass of the carboxylic ester hydrolases (EC 3.1.1.1) which hydrolyse ...
G, Williamson, C B, Faulds, P A, Kroon
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Occurrence, properties, and applications of feruloyl esterases
Applied Microbiology and Biotechnology, 2009Feruloyl esterases hydrolyze the ester linkages of ferulic and diferulic acids present in plant cell walls. This interesting group of enzymes also has a potentially broad range of applications in the pharmaceutical and agri-food industries. An overview of the current knowledge of fungal feruloyl esterases focusing on the diverse of substrate ...
Takuya, Koseki +4 more
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Functional classification of the microbial feruloyl esterases
Applied Microbiology and Biotechnology, 2004Feruloyl esterases have potential uses over a broad range of applications in the agri-food industries. In recent years, the number of microbial feruloyl esterase activities reported has increased and, in parallel, even more related protein sequences may be discerned in the growing genome databases.
V F, Crepin, C B, Faulds, I F, Connerton
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Feruloyl esterase activity and its role in regulating the feruloylation of maize cell walls
Plant Physiology and Biochemistry, 2020Cell walls of grasses have ferulic acid (FA) ester-linked to the arabinosyl substitutions of arabinoxylan (AX). Feruloyl esterases (FAE) are carboxylic acid esterases that release FA from cell walls and synthetic substrates. Despite the importance of FA for cell wall recalcitrance and in response to biotic and abiotic stresses, the physiological ...
Dyoni M, Oliveira +8 more
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Characterization of feruloyl esterases in maize pollen
Planta, 2019Ferulic acid esterases have been identified and partially purified from maize pollen. Results suggest that maize pollen FAEs may play an important role in pollen fertilization. A critical step in maize (Zea mays) seed production involves fertilization of the ovule by pollen, a process that relies on ability of the pollen tube to grow through the highly
Marcia M, de O Buanafina +4 more
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A halotolerant type A feruloyl esterase from Pleurotus eryngii
Fungal Biology, 2014An extracellular feruloyl esterase (PeFaeA) from the culture supernatant of Pleurotus eryngii was purified to homogeneity using cation exchange, hydrophobic interaction, and size exclusion chromatography. The length of the complete coding sequence of PeFaeA was determined to 1668 bp corresponding to a protein of 555 amino acids.
Diana Linke +2 more
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Regioselective esterase-catalyzed feruloylation of l-arabinobiose
Carbohydrate Research, 2006The regioselective chemoenzymatic synthesis of O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl]-(1-->5)-L-arabinofuranose has been achieved. The reaction parameters affecting the feruloylation rate and conversion of the enzymatic synthesis, such as the composition of the reaction medium, substrate and enzyme concentration, have been investigated.
Christina, Vafiadi +2 more
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Applied Microbiology and Biotechnology, 2012
Ferulic acid (FA) is a component of plant cell walls that has applications in food, cosmetic, and health products, but its applications are limited by its high insolubility. We synthesized water-soluble FA derivatives by esterification of FA with diglycerol (DG) using feruloyl esterase purified from a commercial enzyme preparation produced by ...
Masaki, Kikugawa +3 more
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Ferulic acid (FA) is a component of plant cell walls that has applications in food, cosmetic, and health products, but its applications are limited by its high insolubility. We synthesized water-soluble FA derivatives by esterification of FA with diglycerol (DG) using feruloyl esterase purified from a commercial enzyme preparation produced by ...
Masaki, Kikugawa +3 more
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A Zymogram technique for preliminary screening and characterization of feruloyl esterases
Enzyme and Microbial Technology, 2017We report here, for the first time, a zymogram technique designed for rapid screening of feruloyl esterase using ethyl ferulate as enzyme substrate and casein precipitation as enzyme activity indicator.
Sakthi Kumaran, Palaniswamy +1 more
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Metagenomic discovery of feruloyl esterases from rumen microflora
Applied Microbiology and Biotechnology, 2019Feruloyl esterases (FAEs) are a key group of enzymes that hydrolyze ferulic acids ester-linked to plant polysaccharides. The cow's rumen is a highly evolved ecosystem of complex microbial microflora capable of converting fibrous substances to energy. From direct cloning of the rumen microbial metagenome, we identified seven active phagemids conferring ...
Dominic W. S. Wong +2 more
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