Results 51 to 60 of about 894,095 (288)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

FEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source

Thermomechanical Fatigue and Creep–Fatigue Interaction of Inconel 718 Additively Manufactured by Laser Beam Powder Bed Fusion

Advanced Engineering Materials, EarlyView.
Thermomechanical fatigue tests of laser beam powder bed fusion (PBF‐LB) Inconel 718 show that the additively manufactured material reaches almost the lifetimes of conventionally‐rolled material under no‐dwell conditions. Introducing dwell times at the maximum temperature markedly reduces the lifetimes due to pronounced grain boundary sliding associated
Stefan Guth, Tomáš Babinský, Jan Lars Riedel, Wen Hao Kan, Aijun Huang, Martin Heilmaier, Ivo Šulák   +6 more
wiley   +1 more source

A Chaotic State in the Transition From Single Toward Multiple Pulse for Soliton Fiber Lasers

IEEE Photonics Journal, 2020
High-order soliton in anomalous-dispersion ultrafast fiber lasers is rarely researched due to its intrinsic instability in dissipative systems. For the first time, we numerically demonstrate the ultrafast pulse in fiber lasers will deviate from the ...
Yueqing Du, Mengmeng Han, Xuewen Shu
doaj   +1 more source

Is an Apple an Orange? A Large Language Model Benchmark for Candidate Term Extraction and Subclass Decisions Against Upper Ontologies in Engineering and Materials Science

Advanced Engineering Materials, EarlyView.
Building machine‐readable vocabularies for materials science is slow, expert‐driven work. This study benchmarks 13 large language models on two of its first steps: finding candidate terms in engineering articles and deciding where they belong in a class hierarchy.
Thomas Bjarsch, Maximilian Holland, Klaus Drechsler, Johannes Schilp   +3 more
wiley   +1 more source

Linewidth and phase noise characteristics of DFB fibre lasers

, 2004
The anomalous linewidth behavior in a DFB fiber laser is investigated. It is shown that not only does the linewidth deviate drastically from the Schawlow-Townes linewidth formula by increasing with pump and laser power, but it also varies significantly ...
Voo, N.Y., Horak, P., Morten Ibsen, N. Y. Voo, Ibsen, M., Peter Horak, Wei H. Loh, Loh, W.H.   +7 more
core   +1 more source

Rains of solitons in a fiber laser

Optics Express, 2009
We report a novel and intriguing nonlinear dynamics observed in a fiber laser cavity, in which soliton pulses are created from an extended noisy background and drift until they reach a condensed phase comprising several tens of aggregated solitons. This soliton flow can be adjusted with manual cavity tuning, and can even be triggered by the injection ...
Chouli, Souad, Grelu, Philippe
openaire   +3 more sources

Current Status and Challenges in Data Collection for Aerospace Coatings Deposited by Plasma Spraying

Advanced Engineering Materials, EarlyView.
An innovative approach has been integrated into the GRENAT project to optimize plasma spraying and coating performance. Raw materials are accelerated and melted in the plasma generated by torches, creating coatings. Monitoring sensors collect process data which are combined with ex situ characterization data.
Lila Randriamananjara, Vincent Rat, Quentin Dubreuil, Patrice Valorge, Simon Goutier, Alain Denoirjean, Olivier Messe, Céline Ruelle, Jean‐Philippe Poli   +8 more
wiley   +1 more source

Graphene-enabled sub-picosecond solitons in mode-locked mid-infrared fluoride fiber lasers [PDF]

EPJ Web of Conferences
We report on sub-picosecond pulses generation from mode-locked erbium-doped-fiber lasers operating at 2.8 µm and 3.5 µm using graphene-based saturable absorbers. The lasers produce stable 600 fs pulses at 2.8 µm and 700 fs at 3.5 µm.
Hatim Saad, Eremeev Kirill, Bae JiEun, Loiko Pavel, Idlahcen Saïd, Hanzard Pierre-Henry, Godin Thomas, Berthelot Thibaud, Cozic Solenn, Poulain Samuel, Camy Patrice, Hideur Ammar   +11 more
doaj   +1 more source

Investigating the Effect of Inclination Angle and the Influence of Scan Parameters on the Appearance and Thermal and Mechanical Properties of Filigree Tension Rods of LPBF‐Manufactured NiTi Shape‐Memory Alloys

Advanced Engineering Materials, EarlyView.
This article investigates the dependence of the properties on the manufacturing orientation in the form of the inclination angle to the build plate of LPBF‐fabricated NiTi rods with a diameter of 150–220 µm. Metallographic, chemical, thermal, and mechanical characterization show behavior under identical manufacturing conditions that ranges from ...
Sandra Herzig, Michael Werner, Christian Rotsch   +2 more
wiley   +1 more source