Results 41 to 50 of about 61,629 (267)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Early‐life high‐fat diet exposure increases Achilles tendon stiffness and induces transcriptomic alterations

FEBS Open Bio, EarlyView.
Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin, Ziyang Yuan, Wenli Jiang, Ai Guo   +3 more
wiley   +1 more source

Hydrostatic pressure activates HIF‐1α via β‐catenin to promote stemness in breast cancer cells

FEBS Open Bio, EarlyView.
To mimic the elevated intestinal fluid pressure in breast cancers, we loaded human breast cancer cells (MCF‐7, MDA‐MB‐453, and BT‐474) to 50 mmHg hydrostatic pressure. Hydrostatic pressure exposure upregulated HIF‐1α and induced stemness in MCF‐7 and BT‐474 cells.
Da Zhai, Yong Xu, Jingjing Ma, Ratchanee Duangrat, Chen Yan, Jingyan Liang, Hua Wei, Bin Jiang, Tao‐Sheng Li   +8 more
wiley   +1 more source

Patrones de variación de la densidad de la madera y morfometría celular de Salix babylonica para la determinación de la edad de transición entre madera juvenil y madura

Maderas: Ciencia y Tecnología, 2014
El objetivo del trabajo fue analizar los patrones de variación axial y radial de la densidad y morfometría celular de la madera de sauce americano y determinar la edad de transición de madera juvenil a madura.
Ana C. Cobas, María C. Area, Silvia Monteoliva   +2 more
doaj  

Alkali-treated coir fibre-pith composite for waste water treatment

Journal of Industrial Textiles, 2022
Coir fibre and pith are eco-friendly material used in the preparation of composites. Coir fibre and coir pith were treated with sodium hydroxide to study the effect of alkali for prolonged exposure on its properties.
Monnisha Ganesan, Gobi Nallathambi
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Transición de madera juvenil a madura en un clon de Populus deltoides implantado en Buenos Aires Argentina

Maderas: Ciencia y Tecnología, 2014
El objetivo del trabajo fue determinar la edad de transición de madera juvenil a madura a partir de las propiedades de la madera en un clon de álamo.
Ana C. Cobas, María C. Area, Silvia Monteoliva   +2 more
doaj  

MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis

FEBS Open Bio, EarlyView.
MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss, Ao Li, Eduardo H. Gilglioni, Mayank Bansal, Sumeet Pal Singh, Latifa Bakiri, Alessandra K. Cardozo, Esteban N. Gurzov   +7 more
wiley   +1 more source

Transición de madera juvenil a madura en un clon de Populus deltoides implantado en Buenos Aires Argentina Juvenile to mature wood transition in one clone of Populus deltoides implanted in Buenos Aires, Argentine

Maderas: Ciencia y Tecnología, 2013
El objetivo del trabajo fue determinar la edad de transición de madera juvenil a madura a partir de las propiedades de la madera en un clon de álamo.
Ana C Cobas, María C Area, Silvia Monteoliva   +2 more
doaj  

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source