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Flow Cytometry for Filamentous Fungi

2020
Flow cytometry is a powerful high-throughput method, which enables a fast and multi-parameter analysis of single cells and particles. A plethora of different dyes for flow cytometry are available to label different parts of a cell in addition to in vivo markers like fluorescent proteins.
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Population genetics of filamentous fungi

Antonie van Leeuwenhoek, 1994
Population genetics aims to understand causes and consequences of the genetic structure of populations, i.e. distributions of genetic variants in space and time. Among the most important determinants of the genetic population structure is the genetic system itself, which is the collection of processes and mechanisms responsible for the transmission of ...
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Protoplast Transformation of Filamentous Fungi

2010
The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed.
B Gillian, Turgeon   +3 more
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Transposable Elements in Filamentous Fungi

Annual Review of Microbiology, 2003
▪ Abstract  The past 10 years have been productive in the characterization of fungal transposable elements (TEs). All eukaryotic TEs described are found including an extraordinary prevalence of active members of the pogo family. The role of TEs in mutation and genome organization is well documented, leading to significant advances in our perception of
Marie-Josée, Daboussi, Pierre, Capy
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Biosorption of nickel using filamentous fungi

Applied Biochemistry and Biotechnology, 1998
Nickel (Ni) uptake capability from aqueous solutions was studied in a filamentous fungi strains group of Rhizopus sp., Penicillium sp. Aspergillus sp., Trichoderma sp., Byschoclamyss sp., and Mucor sp. The metal uptake of a Rhizopus sp. strain, which has the highest uptake capacity, was corroborated by electron microscopy; no Ni deposits were observed ...
L, Mogollón   +4 more
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Supernumerary chromosomes in filamentous fungi

Current Genetics, 1998
Within a fungal species, a subset of individuals may have more than the minimal complement of chromosomes. If the extra chromosomes are composed primarily of DNA not found in all representatives of the species, they are most appropriately referred to as supernumerary chromosomes.
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Filamentous Fungi in Cheese Production

2018
Filamentous fungi play important roles in the production of a variety of cheeses. The most famous are the blue cheeses, such as Roquefort or Gorgonzola, in which Penicillium roqueforti is the principal mold, and the moldy soft cheeses, such as Camembert or Brie, in which production involves Penicillium camemberti. There are also other filamentous fungi
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Chromosome visualisation in filamentous fungi

Journal of Microbiological Methods, 2006
Many attempts have been made to study the chromosomes of fungi, but a major problem is that fungal nuclei are so small. Fungal chromosomes are at the lowest resolution of light microscopy; thus few attempts to visualise fungal chromosomes have been successful. Fungi examined have been mainly Ascomycotina.
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Proteome Studies of Filamentous Fungi

2011
The continued fast pace of fungal genome sequence generation has enabled proteomic analysis of a wide variety of organisms that span the breadth of the Kingdom Fungi. There is some phylogenetic bias to the current catalog of fungi with reasonable DNA sequence databases (genomic or EST) that could be analyzed at a global proteomic level.
Scott E, Baker, Ellen A, Panisko
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Vacuoles in Filamentous Fungi

2014
Using the ectomycorrhizal fungus Pisolithus tinctorius, four zones of vacuolar morphology from the growing hyphal tip were observed: (i) the apical zone, which has few or no vacuoles; (ii) the subapical zone with small ovoid-spherical vacuoles; (iii) the nuclear zone, where tubular vacuoles predominate; and (iv) the basal zone, where large spherical ...
Emma Jean Bowman, Barry J. Bowman
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