Results 61 to 70 of about 78,905 (259)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

Evaluation of Wood and Cellulosic Materials as Fillers in Artificial Diets for Lyctus africanus Lesne (Coleoptera: Bostrichidae)

open access: yesInsects, 2015
We studied the usefulness of wood- and cellulose-based diets for L. africanus Lesne. Three diets were prepared which differed on the base ingredients; wood particles (Diet 1), cellulose powder (Diet 2), and alpha-cellulose (Diet 3).
Titik Kartika, Tsuyoshi Yoshimura
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

open access: yesFEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane   +11 more
wiley   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

open access: yesFEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia   +3 more
wiley   +1 more source

Tertiary care centres must do more for patients with unknown conditions: Lessons learned from a child

open access: yesPatient Experience Journal, 2018
Postural Orthostatic Tachycardia Syndrome (POTS) is a debilitating disease with a commonly non-uniform and unspecific presentation that makes diagnosis difficult.
Guido Filler, Lana Rothfels
doaj  

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

open access: yesFEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong   +3 more
wiley   +1 more source

Shaping lips with fillers

open access: yesJournal of Cutaneous and Aesthetic Surgery, 2015
The lips and the eyes enhance facial beauty, and they have been highlighted since time immemorial. Rejuvenating the lips with fillers, frequently hyaluronic acid (HA), is a common procedure but requires expertise. The objective of this text is to describe the procedure in detail and cover the practical aspects of injecting lips with fillers.
openaire   +2 more sources

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

open access: yesFEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata   +13 more
wiley   +1 more source

Heat-resistant ferrochrome slag based concrete

open access: yesИнженерно-строительный журнал, 2014
In the construction materials industry, expensive refractories (fire clay, silica, high- aluminous, etc.), are used as the main lining materials which despite their high fire resistance are inefficient for the use in thermal aggregates with an operation ...
M.T. Zuginisov   +3 more
doaj   +1 more source

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