Results 141 to 150 of about 3,247 (253)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

FAIR digital twins for biodiversity: enabling data, model, and workflow integration. [PDF]

NPJ Biodivers
Islam S, Koivula H, Andrew C, Gordillo JL, Weiland C, Schigel D, Endresen D, Arvanitidis C, Chadwick E, Soiland-Reyes S.   +9 more
europepmc   +1 more source

Hydrostatic pressure activates HIF‐1α via β‐catenin to promote stemness in breast cancer cells

FEBS Open Bio, EarlyView.
To mimic the elevated intestinal fluid pressure in breast cancers, we loaded human breast cancer cells (MCF‐7, MDA‐MB‐453, and BT‐474) to 50 mmHg hydrostatic pressure. Hydrostatic pressure exposure upregulated HIF‐1α and induced stemness in MCF‐7 and BT‐474 cells.
Da Zhai, Yong Xu, Jingjing Ma, Ratchanee Duangrat, Chen Yan, Jingyan Liang, Hua Wei, Bin Jiang, Tao‐Sheng Li   +8 more
wiley   +1 more source

Ten quick tips for using the NIH Comparative Genomics Resource. [PDF]

PLoS Comput Biol
Tvedte ES, Arighi C, Carson MB, Rasmussen LV, Holmes K, Murphy TD.   +5 more
europepmc   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

Data Notes: A New Article Type for Preserving and Sharing Biodiversity Data. [PDF]

Ecol Evol
Soares BE, Jenkins GB.
europepmc   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

A framework for reproducibly managing coupled research software and data assets based on shared transformation functions. [PDF]

Patterns (N Y)
Kuckertz P, Fuchs B, Schönau J, Gardian H, Knosala K, Arellano Ruiz ES, Göpfert J, Gils HC, Weinand JM, Jochem P, Linßen J, Stolten D.   +11 more
europepmc   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

CLUES A Comprehensive Workflow for Integrating Geospatial Data in Biomedical Research. [PDF]

Nat Commun
Jentsch M, Polemiti E, Renner P, Hese S, Schepanski K, Eils R, Marquand A, Twardziok S, Schumann G, environMENTAL consortium.   +9 more
europepmc   +1 more source