Results 51 to 60 of about 5,851 (257)

Epigenetic blind spots – the role of DNA methylation dynamics in stem cell‐based models of embryogenesis

FEBS Letters, EarlyView.
Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil, Anna Corrà, Annalisa Scapolatiello, Chengxiang Qiu, Gianluca Amadei   +4 more
wiley   +1 more source

Effect of granular waste compact disc on bond strength between steel bars and surrounding concrete

Fracture and Structural Integrity
In recent decades, researchers have tried to use waste materials as a partial or complete replacement of the gravel ingredients of concrete to reduce environmental impact. One of these materials is electronic waste. The problem of this disposal material,
Barzan Omar Mawlood, Sanaa Ismael Khaleel, Ahmed Heidayet Mohammad   +2 more
doaj   +1 more source

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

The effect of replacing aggregates with oyster shell powder on the performance of asphalt concrete

Materiales de Construccion
In response to the global trend towards achieving net zero emissions, various industries are actively promoting the circular economy. Central to this goal is the strategic utilization of alternative materials to replace virgin materials, thereby ...
J.R. Chang, Y.Y. Lo
doaj   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Balancing Mechanical Strength and Hydraulic Functionality of Fine Aggregate Free Pervious Concrete Using Expanded Vermiculite

Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi
Pervious concrete is widely used for sustainable pavement applications due to its ability to reduce surface runoff and enhance groundwater recharge; however, its application is often limited by low mechanical strength caused by the absence of fine ...
Rahmad Pasca Try Valent, Lintang Dian Artanti   +1 more
doaj   +1 more source

DEVELOPMENT OF SUSTAINABLE CONCRETE USING COPPER SLAG AS A SUBSTITUTE OF FINE AGGREGATE

, 2023
Abstract The research aimed to investigate the mechanical and durability qualities of concrete mix designs by incorporating copper slag (CS) as a substitute for fine aggregate of natural sand (NS). Eleven concrete mixtures were casted, including the control mix, with varying concentrations of CS ranging from 0% to 100% at an increment of 10%.
AMRUTA A. YADAV, AJAY R. GAJBHIYE, ALPESH A. ADEKAR, ROSHAN R. UMATE   +3 more
openaire   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source