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FISH Methods in Cytogenetic Studies

2013
This chapter describes the various methods derived from the protocol of standard fluorescent in situ hybridization (FISH) that are used in human, animal, plant, and microbial studies. These powerful techniques allow us to detect and physically map on interphase nuclei, chromatin fibers, or metaphase chromosomes probes derived from single-copy genes to ...
Miguel, Pita   +5 more
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Cytogenetic techniques in fish genetics

Journal of Fish Biology, 1985
A modification of a previously described method of fish blood lymphocyte culture is described and discussed with the results of various chromosome banding protocols as applied to fish chromosomes.
S. E. Hartley, M. T. Horne
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Cytogenetic and FISH analysis of endometrial carcinoma

Cancer Genetics and Cytogenetics, 1994
Endometrial cancer is a common gynecologic tumor, yet reports of cytogenetic studies are few. We studied chromosomes from seven primary specimens of endometrial cancer. Six had abnormal chromosomes; five had a diploid-hyperdiploid modal number and one was triploid. One specimen had a normal karyotype.
N K, Shah   +6 more
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FISH in clinical cytogenetics

2002
Abstract In recent years the possibilities for visualizing several chromosomal targets simultaneously has meant that FISH analysis has an increasing role to play in the study of patient samples. The clinical cytogenetics service laboratory no longer focuses on the morphological analysis of chromosomal aberrations alone, but now has to ...
Jeremy A Squire, P Marrano, E Kolomietz
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[FISH, cytogenetic analysis].

Rinsho byori. The Japanese journal of clinical pathology, 2001
We describe here the clinical applications of fluorescence in situ hybridization(FISH) in patients with chronic myelogenous leukemia under treatment with interferon-alpha, some cases with a false positive caused by preparation of specimen and/or probes, and the detection of chimerism after sex-mismatched bone marrow transplantation based on our ...
S, Miyanishi, A, Okumura
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CYTOGENETICS OF CHILEAN FISHES: A COMMENTED DATABASE

BIOCYT Biología Ciencia y Tecnología, 2020
<p>A database containing data from cytogenetic studies of Chilean fish species is documented for the first time. The cytogenetic data compiled for Chilean fishes include 28 species belonging to 11 families, 9 orders and 16 genera, taking as reference 18 publications since 1972.
Pedro Jara-Seguel   +3 more
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Cytogenetic and FISH investigations on tetrasomy 8 in ANLL

Cancer Genetics and Cytogenetics, 1995
Two patients with "de novo" ANLL and tetrasomy of chromosome 8 at diagnosis are described. A mosaic karyotype with coexistence of normal metaphases was found in both cases. Trisomy 8 was also present in one metaphase of the first patient. Fluorescent in situ hybridization with a centromeric probe from chromosome 8 was applied in the second case ...
LA STARZA, Roberta   +4 more
openaire   +3 more sources

Mouse cytogenetics and FISH

1999
Abstract There are a several situations in which it may be helpful to look directly at mouse chromosomes. One may wish to screen a specific living mouse for possible chromosome rearrangements, or to determine precise cytogenetic breakpoints without compromising the ability of the mouse to breed.
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Fish Cytogenetic Research: Advances, Applications and Perspectives

Netherlands Journal of Zoology, 1991
Methods developed since 1976 for harvesting, preparing and banding fish chromosomes are now commonly used for taxonomic and phylogenetic studies, genetic control and chromosome manipulations in fish breeding and in monitoring aquatic pollutants by examining chromosomal aberrations.
Ozouf-Costaz, C., Foresti, F.
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