High-Resolution CSRR-Based Microwave Sensor for Soil Moisture Content Monitoring. [PDF]
Sensors (Basel)Alduwish S +4 more
europepmc +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Quantitative Guanidinium CEST-Based pH Mapping at 3 T in Healthy and Pathological Muscle. [PDF]
NMR BiomedHenriet V +4 more
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
A Stacking-Enhanced Support Vector Regression Model for Predicting the Hot Deformation Flow Stress of TC18 Alloy. [PDF]
Materials (Basel)Jiang X +5 more
europepmc +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Deciphering the Combined Effects of Hydroxysafflor Yellow A and Calycosin Through Coupled PK-PD Modeling. [PDF]
Biology (Basel)Hu Y, Zhao X, Jin W, Yu L.
europepmc +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Precise Measurements of the Absolute Nonlinear Refractive Index of Fused Silica Using High-Repetition-Rate Femtosecond Laser and Nonlinear Ellipse Rotation Signal. [PDF]
ACS OmegaMoysés RM +5 more
europepmc +1 more source