“White and Clear” (WaC): A tunable hydrophilic tissue clearing strategy for multi‐species 3D imaging
VIEW, EarlyView.Optical tissue clearing methods help scientists make tissues transparent to image structures deep inside organs in 3D, but many existing techniques are limited in performance and difficulty. In this study, we developed an optimized clearing method called WaC (White and Clear), which improves upon existing techniques by combining efficient tissue ...
Vicente Llorente +12 more
wiley +1 more source
The effect of chemical fixation with paraformaldehyde, glutardialdehyde or methanol on immunofluorescence staining of neutrophils and neutrophil extracellular traps. [PDF]
Innate ImmunPilchová V +4 more
europepmc +1 more source
Assessing the accuracy of field‐based versus laboratory methods for determining the age of roe deer
Wildlife Biology, EarlyView.Accurate age determination in roe deer Capreolus capreolus is essential for selective harvesting and informed population management, yet field‐based methods widely used by hunters remain imprecise. This study compared field‐based and laboratory age determination methods in 204 harvested roe deer, categorized by sex and habitat type (forest versus open ...
Michael Manton +4 more
wiley +1 more source
Reimagining cytopathology in the molecular era: Integration or fragmentation? [PDF]
CytojournalDas S, Kumar RN, Dey B, Kalita P.
europepmc +1 more source
Extraocular Photoreception in Optic Lobes, Suckers, and Skin of Octopus vulgaris
Integrative Zoology, EarlyView.Evidence of extra‐ocular photoreception in Octopus vulgaris (a) Diagram of the O. vulgaris different tissues considered: SPB, sucker proximal big; SPL, sucker proximal large; SM, sucker medium; SD, sucker distal; SK, skin; OL, optic lobes; RT, retina; (b‐d) Gene expression analysis of Ov‐GRK1 (red), Ov‐retinochrome (green), Ov‐rhodopsin (blue) mRNA ...
Valeria Maselli +7 more
wiley +1 more source
A new efficient immunoprotocol to detect chromosomal/nuclear proteins along with repetitive DNA in squash preparations of formalin-fixed, long-stored root tips. [PDF]
Plant MethodsGolczyk H.
europepmc +1 more source
Integrative Zoology, EarlyView.Brain size may be influenced by the cognitive demands of sociality (social brain hypothesis). We used microCT to compare CNS and brain volumes in social versus solitary huntsman and crab spiders. Social huntsman spiders had larger arcuate and mushroom bodies, while social crab spiders had larger visual neuropils.
Vanessa Penna‐Gonçalves +6 more
wiley +1 more source
New dimensions in acidocalcisome research: the potential of cryo-EM to uncover novel aspects of protozoan parasite physiology. [PDF]
mBioAugusto I +6 more
europepmc +1 more source
Integrative Zoology, EarlyView.High altitudes are challenging for the animals that inhabit these environments. The Xizang plateau frog (Nanorana parkeri) survives in high‐altitude environments through a synergistic strategy of metabolic remodeling and protein expression adjustment to optimize energy efficiency and enhance cellular protection.
Xuejing Zhang +6 more
wiley +1 more source