Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
<i>Listeria monocytogenes</i> serotype 4b strains demonstrate a fitness advantage over strains of serotypes 1/2a and 4b-v1 on cantaloupe rind and flesh at different temperatures. [PDF]
Gorski L, Aviles Noriega A, Carter MQ.
europepmc +1 more source
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source
Optimizing dietary protein and lipid levels for better growth performance, nutrient retention and flesh quality of grow-out striped catfish (Pangasianodon hypophthalmus). [PDF]
Prakash P +7 more
europepmc +1 more source
Overview of molecular signatures of senescence and associated resources: pros and cons
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas +6 more
wiley +1 more source
Mechanical Resistance of New Apple Genotypes for Automated Harvesting. [PDF]
Císler M +5 more
europepmc +1 more source
Validation of a portable three-dimensional imaging system for volumetric measurement in the periorbital region. [PDF]
Zhang X +6 more
europepmc +1 more source
Analysis of Genotypic Variations in the Anthocyanin Biosynthetic Pathway in Potatoes. [PDF]
Lee CM +6 more
europepmc +1 more source

