Results 61 to 70 of about 139 (134)
This research demonstrates real‐time visualization of supramolecular sol‐gel transitions using Fluorescence Lifetime Imaging Microscopy (FLIM). By leveraging 5D visualization, integrating 3D space, time, and polarity resolution, we achieved maskless sub‐micrometric photopatterning within the gel matrix.
Federico Movilla +5 more
wiley +1 more source
A Triangulenum‐Based Biosensor Platform for Mapping Kinase Activity in T Cells
Novel peptide biosensors based on long‐lifetime triangulenium dyes are developed for the real‐time, non‐invasive mapping of Akt1 and Src kinase activities in living cells. Utilizing FLIM microscopy, these highly specific, biocompatible probes overcome cellular autofluorescence.
Carmen Salto‐Giron +11 more
wiley +1 more source
Abstract Multimodal optical microscopy has recently converged with artificial intelligence (AI) to enable in silico labelling—the computational prediction of fluorescence‐like molecular contrast from label‐free measurements. Fluorescence plays a crucial role in linking microscopy and spectroscopy at molecular level, enabling image formation by means of
A. Diaspro +9 more
wiley +1 more source
Tip Quantification enables quantitative analyses over versatile selections of regions of interest. Summary Cell polarity and tip growth rely on the dynamic spatial organization of signaling and structural components. Quantitative characterization of these spatiotemporal dynamics is critical for understanding polarized cell growth, yet manual ...
Jingzhe Guo +7 more
wiley +1 more source
SUMMARY SQUAMOSA PROMOTER BINDING PROTEIN‐LIKE 8 (SPL8) is a plant‐specific transcription factor essential for anther development, but its regulatory partners and downstream mechanisms remain largely unknown. We identified 26 SPL8‐interacting transcription factors by yeast two‐hybrid screening and bimolecular fluorescence complementation (BiFC) assays,
Tingting Li, Lei Zhang, Shuping Xing
wiley +1 more source
Abstract In recent years there has been growing interest in bracken toxins, in understanding their effect, occurrence and prevention. Most of these substances are glycosides and are therefore highly water soluble and mobile. They can transfer to drinking water or via livestock to milk and meat, thereby potentially posing a health risk.
Hans Reinhard +3 more
wiley +1 more source
Nanorulers for Super‐Resolution Microscopy
Nanorulers serve as essential calibration standards to validate optical super‐resolution microscopy down to the Ångström scale. Complementary DNA‐, protein‐, and virus‐based scaffolds provide unique strategies to benchmark spatial resolution, evaluate quantitative labeling efficiency, and mini‐mize linkage errors.
Gerti Beliu +5 more
wiley +1 more source
FLIM Networks with Bag of Feature Points
Accepted at the 28th Iberoamerican Congress on Pattern Recognition (CIARP 2025).
Martinelli, J.D. +5 more
openaire +3 more sources

