Genetic diversity and population structure of U.S. Suffolk sheep participating in the national sheep improvement program. [PDF]
Wilson CS +7 more
europepmc +1 more source
Forest fragmentation in an African biodiversity hotspot impacts mixed-species bird flocks
N. Cordeiro +5 more
semanticscholar +1 more source
Backyard poultry flocks and salmonellosis: a recurring, yet preventable public health challenge.
C. B. Behravesh +3 more
semanticscholar +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
A multiplex TaqMan real-time PCR assay for differential identification of wild-type and vaccine strains of Mycoplasma gallisepticum. [PDF]
Xin J +16 more
europepmc +1 more source
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
Fowl adenovirus infection and inclusion body hepatitis in Canada: genotyping trends from 2008 to 2024. [PDF]
Ojkić D +7 more
europepmc +1 more source
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Understanding sheep lameness management in relation to culling and the UK five-point plan. [PDF]
Clark B, Mahon N.
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source

