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Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source
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On the evolution of internet flow characteristics

Proceedings of the Applied Networking Research Workshop, 2021
The ongoing evolution of technologies and network services on the Internet indicates ongoing changes in traffic and flow characteristics. Since the analysis of flow characteristics, like duration, size, and rate, has been a frequently studied topic before, results on the evolution of flow characteristics are rare.
Simon Bauer   +4 more
openaire   +1 more source

Flow Characteristics of Aortic Cannulae

Journal of Cardiac Surgery, 1995
Atheroembolism from the ascending aorta is an emerging cause of noncardiac complications after open heart surgery. We designed a new arterial cannula specifically to reduce the exit force and velocity of blood flow, thereby reducing the "sandblasting" effect of the exiting blood jet. The cannula has a closed tip and an internal cone that diffuses blood
D D, Muehrcke   +3 more
openaire   +2 more sources

Electrical Characteristics of Flowing Blood

IEEE Transactions on Biomedical Engineering, 1979
Erythrocyte orientation, deformation, and axial accumulation cause differences in resistance between flowing and resting blood. Through theoretical calculations and experimental measurements, we studied the effects of these factors and of sinusoidal and pulsatile flow on blood resistivity.
K, Sakamoto, H, Kanai
openaire   +2 more sources

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