Results 241 to 250 of about 20,769,954 (368)

Expression and purification of E140 protein antigen fragments of Plasmodium vivax and Plasmodium berghei for serological assays

open access: yesFEBS Open Bio, EarlyView.
We provide a step‐by‐step guide for producing E140 antigen fragments from Plasmodium berghei (Pb1) and Plasmodium vivax (Pv1). Pb1/Pv1 are expressed in E. coli, solubilized by freeze–thawing, refolded by slow dilution, purified by affinity chromatography (IMAC), then concentrated and subjected to quality control.
Rodolfo Ferreira Marques   +5 more
wiley   +1 more source

The clinical spectrum of SMA‐PME and in vitro normalization of its cellular ceramide profile

open access: yesAnnals of Clinical and Translational Neurology, Volume 9, Issue 12, Page 1941-1952, December 2022., 2022
Abstract Objective The objectives of this study were to define the clinical and biochemical spectrum of spinal muscular atrophy with progressive myoclonic epilepsy (SMA‐PME) and to determine if aberrant cellular ceramide accumulation could be normalized by enzyme replacement.
Michelle M. Lee   +16 more
wiley   +1 more source

Optimization research on battery thermal management system based on PCM and mini-channel cooling plates

open access: yesCase Studies in Thermal Engineering
A phase change materials (PCM) coupled mini-channel cooling plates model was established for the optimization of the battery thermal management system (BTMS). Specific cooling strategies with different discharge rates of the battery were discussed. For a
Honglei Ren   +3 more
doaj  

CD44v, S1PR1, HER3, MET and cancer‐associated amino acid transporters are promising targets for the pancreatic cancers characterized using mAb

open access: yesFEBS Open Bio, EarlyView.
Novel mAbs could detect cancer‐associated membrane proteins on various type of pancreatic ductal adenocarcinomas (PDAC) by flow cytometry with viable PDAC cells and by immunohistochemistry with PDAC tissues. Results of protein expression were substantiated by mRNA expression by The Cancer Genome Atlas.
Takashi Nakano   +16 more
wiley   +1 more source

Enhanced flow boiling heat transfer with three-section sudden expansion microchannels

open access: yesCase Studies in Thermal Engineering
In this study, a three-section sudden expansion microchannels (TSE-MCs) heat sink was proposed to stabilize the flow boiling and improve the thermal performance of the heat sink exposed to high-heat-flux scenarios. The structural effect of newly designed
Zhiqiang Zhang   +4 more
doaj  

Flow control in a laminate capillary-driven microfluidic device. [PDF]

open access: yesAnalyst, 2021
Jang I   +5 more
europepmc   +1 more source

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