Results 181 to 190 of about 3,078,576 (239)

A cascade synthesis of 2-aminothiochromones from 2-fluorophenyl ketone and thiocarbonyldiimidazole. [PDF]

Beilstein J Org Chem
Zhang K, Shao H, Liao K, Xu Y, Du S, Zhao Y, Li G, Xu W, Huang H, Li P.   +9 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Waste to Alternative Fuel: Experimental Investigation of the Effects of Pyrolytic Oil Obtained from Waste Tires, Waste Transformer Oil, and Diesel Fuel Ternary Blends on Engine Performance and Exhaust Emissions in a Diesel Engine. [PDF]

ACS Omega
Yesilyurt MK, Öner İV, Doğan TH, Arpa O, İnce MK.   +4 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Hydrotreated vegetable oil migrates through soil and degrades faster than fossil diesel and hydrotreated vegetable oil-fossil diesel blend. [PDF]

Environ Sci Pollut Res Int
Lahti-Leikas K, Niemistö E, Talvenmäki H, Saartama N, Sun Y, Mercier L, Romantschuk M.   +6 more
europepmc   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

Cognitive control training as an add-on treatment for late-life depression: A multicenter randomized controlled trial. [PDF]

Internet Interv
Vander Zwalmen Y, Vrijsen JN, Meuleman B, Vanderhasselt MA, Naarding P, Collard RM, van Beek M, Oostelbos P, Bolier L, van Driel D, Spijker J, Becker ES.   +11 more
europepmc   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Hydrothermal Liquefaction of Digestate and Copyrolysis of Hydrochar-Straw Blends: An Integrated Approach for Bio-crude and Biochar Production. [PDF]

Energy Fuels
Grottola CM, Marotta G, Amato D, Di Lauro F, Balsamo M, Montagnaro F, Solimene R, Ragucci R, Giudicianni P.   +8 more
europepmc   +1 more source