Results 101 to 110 of about 311,319 (263)
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
COMPOSITE LEVER METHOD FOR POWER FLOW ANALYSIS OF LOOP-TYPE COMPOUND EPICYCLIC GEAR SYSTEM
Due to the complicated structure of the loop-type compound epicyclic gear system, the torque and power relationship between the basic components in the system is complex and variable, forming a variety of power flow types.
XU Lin +5 more
doaj
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
Structure and Hierarchical Control Method of Battery-Based Hybrid Power Flow Controller
This article proposes a battery-based hybrid power flow controller (B-HPFC), offering enhanced flexibility for power flow regulation and energy storage.
Hua Shao +5 more
doaj +1 more source
An In Vitro Blood Flow Loop System for Evaluating the Thrombogenicity of Medical Devices and Biomaterials. [PDF]
Jamiolkowski MA +3 more
europepmc +1 more source
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta +7 more
wiley +1 more source
Gases vacuum dedusting and cooling
Represented are the results of operating the ladle degassing vacuum plant (productivity: 120 tons of liquid steel) with various dust collectors. The process gases’ cooling and dedusting, obtained in the closed loop buran study, provides opportunity to ...
Олексій Олександрович Буров +2 more
doaj +4 more sources
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source
ABSTRACT Advancing artificial intelligence (AI) has transformed learning and work, yet higher education and professional development programs have not systematically equipped learners for AI‐prevalent environments. This lack of preparation creates uncertainty regarding control, responsibility, trust, and accountability.
Moon‐Heum Cho, Jerusalem Merkebu
wiley +1 more source

